Spin trapping reagent
- Highly pure spin trapping reagent
- Contains very low paramagnetic impurities and does not require further purification
- Comes as a crystalline solid for a longer shelf-life
BMPO is a cyclic nitrone spin trap for the specific in vivo or in vitro detection of short-lived hydroxyl, superoxide, and thiyl radicals. BMPO forms distinguishable adducts that can be measured by EPR spectroscopy. Unlike with DMPO (Prod. No. ALX-430-090), the superoxide adduct does not decay into a hydroxyl adduct and has a much longer half-life in cells (t1/2=23min).
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Product Details
| Alternative Name |
5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide |
|---|---|
| Appearance |
White to pinkish crystalline solid. |
| CAS |
387334-31-8 |
| Formula |
C10H17NO3 |
| Identity |
Identity determined by MS and NMR. |
| MW |
199.3 |
| Purity |
≥99% (HPLC) |
| Solubility |
Soluble in DMSO (25mg/ml), 100% ethanol, 100% methanol, and water. |
| Source |
Synthetic |
Handling & Storage
| Use/Stability |
As indicated on product label or CoA when stored as recommended. |
|---|---|
| Handling |
Protect from moisture. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Dynamic Wood Smoke Aerosol Toxicity during Oxidative Atmospheric Aging: S. Wang, et al.; Environ. Sci. Technol. 3, 1246 (2023), Abstract
- Hydrothermal synthesis of PVP-passivated clove bud-derived carbon dots for antioxidant, catalysis, and cellular imaging applications: A.K. Pandey, et al.; Colloids Surf. B Biointerfaces 220, 112926 (2022), Abstract
- Effects of acidity on reactive oxygen species formation from secondary organic aerosols: J. Wei, et al.; ACS Environ. Au. 2, 336 (2022), Abstract
- Activation of peroxymonosulfate by bicarbonate and acceleration of the reaction by freezing: Y.Y. Ah, et al.; Sci. Total Environ. 785, 147369 (2021), Abstract
- Oxidants produced by methylglyoxal-modified collagen trigger ER stress and apoptosis in skin fibroblasts: K. Nowotny, et al.; Free Radic. Biol. Med. 120, 102 (2018), Abstract
- Reactive Oxygen Species Formed by Secondary Organic Aerosols in Water and Surrogate Lung Fluid: H. Tong, et al.; Environ. Sci. Technol. 52, 11651 (2018), Abstract
- Reactive oxygen species formed in aqueous mixtures of secondary organicaerosols and mineral dust influencing cloud chemistry and public health in the Anthropocene: H. Tong, et al.; Faraday Discuss. 200, 251 (2017), Application(s): ROS detection in aqueous mixtures of SOA by applying electron paramagnetic resonance (EPR) spectrometry, Abstract — Full Text
- Oxidation of the Flavonolignan Silybin. In situ EPR Evidence of the Spin-Trapped Silybin Radical: R. Sokolova, et al.; Electrochim. Acta 205, 118 (2016), Application(s): in situ EPR spectroelectrochemistry
- Mechanism of the hydroxyl radical oxidation of methacryloyl peroxynitrate (MPAN) and its pathway toward secondary organic aerosol formation in the atmosphere: T.B. Nguyen, et al.; Phys. Chem. Chem. Phys. 17, 17914 (2015), Application(s): Trapping agent of OH radicals, Abstract — Full Text
- S-allyl cysteine alleviates nonsteroidal anti-inflammatory drug-induced gastric mucosal damages by increasing cyclooxygenase-2 inhibition, heme oxygenase-1 induction, and histone deacetylation inhibition: J.M. Park, et al.; J. Gastroenterol. Hepatol. 29 Suppl. 4, 80 (2014), Application(s): Electron spin resonance (ESR) spectroscopy, Abstract
- Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence: H. Zhao, et al.; PNAS 102, 5727 (2005), Abstract
- Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species: H. Shi, et al.; Arch. Biochem. Biophys. 437, 59 (2005), Abstract
- Spin traps: in vitro toxicity and stability of radical adducts: N. Khan, et al.; Free Radic. Biol. Med. 34, 1473 (2003), Abstract
- Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals: H. Zhao, et al.; Free Radic. Biol. Med. 31, 599 (2001), Abstract
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|---|---|
| Purity | ≥98% (HPLC) |
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|---|---|
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|---|---|
| CAS | 66893-81-0 |
| Purity | ≥99% (HPLC) |
| Alternative Name | 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide, 2-Diethylphosphono-2-methyl-3,4-dihydro-2H-pyrrole 1-oxide, (2-Methyl-3,4-dihydro-1-oxide-2H-pyrrol-2-yl)diethylphosphonate |
|---|---|
| CAS | 157230-67-6 |
| Purity | ≥99% (TLC) |
Last modified: May 29, 2024
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