Alkaline Phosphatase Activity Assay

Alkaline phosphatase (ALP) is an enzyme that catalyzes the hydrolysis of phosphate esters under alkaline conditions with the production of free phosphate. AkrivisBio’s ALP Activity Assay is a simple, sensitive assay to measure ALP activity in a variety of biological samples. In this assay the specific alkaline phosphatase substrate, p-nitrophenyl phosphate is hydrolyzed to phosphate ion and p-nitrophenol. P-nitrophenol formation is then monitored using a spectrophotometer at λmax 405 nm. The absorbance or color intensity is directly proportional to the alkaline phosphatase activity in the sample.