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Protein Analysis
How long may plasma samples be stored (with EDTA and aprotinin or with EDTA-only)? Has this been tested? Is there some degradation of Endothelin-1 while samples are stored?
Plasma samples collected as directed with EDTA and Aprotinin will be stable for at least 1 month at -80°C without…
Tue, Jul 18, 2023
How essential is the addition of aprotinin when collecting blood samples?
The aprotinin is included to help prevent any degradation of ET-1, but also to prevent any further cleavage of the…
Tue, Jul 18, 2023
Which type of plasma samples can be used with your ADI-900-020A, Endothelin-1 ELISA kit (Heparin, EDTA or Citrate)?
The kit has been validated using EDTA plasma samples. Heparin and citrate plasma were not tested. We generally recommend EDTA plasma for…
Tue, Jul 18, 2023
I bought your ADI-900-020A kit and I just realized that the manual indicates to avoid lipemic samples. My plasma samples are lipemic. Is there a way I can try measuring ET-1 concentration with your kit?
How lipid can potentially interfere in the assay has not specifically been tested, but you might want to consider delipidating your…
Tue, Jul 18, 2023
I plan to use your ADI-900-020A kit to measure Endothelin-1 (ET-1) concentration in cell culture supernatant. Samples contain 10% fetal bovine serum (FBS). Will I need to extract them?
The ET-1 ELISA kit is suitable for use with tissue culture supernatants containing up to 10% FBS. In our spike…
Tue, Jul 18, 2023
Which species, other than human, mouse or rat, can these HSP70 ELISA kits detect?
ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101 have not been tested with other species; so, it cannot be said with certainty whether it would be…
Tue, Jul 18, 2023
Which antibodies are used in your HSP70 ELISA kits (ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101)?
This information is considered to be proprietary and cannot be disclosed. The antibody recognizes an internal epitope of HSP70. The…
Tue, Jul 18, 2023
Which ELISA kit should I use to measure the levels of HSP70 in my serum/plasma samples?
The HSP70 ELISA kit (ADI-EKS-700B) was designed to measure HSP70 in cell and tissue lysates, and is not typically sensitive…
Tue, Jul 18, 2023
Will the LTB4 ELISA kit work with serum samples?
This kit has not been validated internally for serum. Sample types that have been validated internally are ETDA plasma, cultured media, saliva…
Tue, Jul 18, 2023
Which species can be analyzed with the LTB4 ELISA kit?
Leukotriene B4 (LTB4) is a small molecule and thus a defined chemical entity that does not change in between species…
Tue, Jul 18, 2023