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Protein Analysis
Can AMP’D® ELISA Signal Amplification Kit ENZ-KIT-100 be used with any commercially available ELISA kit to amplify signals with alkaline phosphatase (AP) substrates?
ANS: AMP'D® ELISA Signal Amplification kit, ENZ-KIT-100 has a signal amplification substrate containing NADPH and enzyme system for increasing the sensitivity…
Tue, Jul 18, 2023
What controls are included in AMPIPROBE® Bacterial Vaginosis Extraction Controls, ENZ-GEN206-0500?
AMPIPROBE® Bacterial Vaginosis Extraction Controls, ENZ-GEN206-0500 are: Negative ExtractionBV Positive Extraction ControlNo BV Target Control
Tue, Jul 18, 2023
How do I choose the correct dilution factor for my samples to be used in my ELISA ?
Optimizing sample preparation and dilution is a key step in the validation of any ELISA assay. It is crucial that…
Tue, Jul 18, 2023
Does the Protein A ELISA kit recognize denatured and non-denatured protein A?
A variety of Protein A constructs have been tested in our Protein A ELISA kit. Please refer to the product…
Tue, Jul 18, 2023
For the Protein A ELISA kit (ADI-900-057) would residual wash buffer affect the assay?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), residual wash buffer can interfere with the assay…
Tue, Jul 18, 2023
For the Protein A ELISA kit, ADI-900-057, would the readings be affected if the wells were to dry out when residual wash buffer is blotted out?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), care should be taken not to allow the…
Tue, Jul 18, 2023
Why do you use protein A affinity columns to purify the antibodies included in ENZ-KIT127, ENZ-KIT128, and ENZ-KIT162 instead of HCP affinity columns?
The main reason is product consistency. Protein A affinity columns have been around for quite a while. They are well…
Tue, Jul 18, 2023
How should I store reconstituted standard, antibody and conjugatefor ADI-900-175?
The standard, antibody and conjugate are single use items and should to be stored at -20°C. Any leftover of reconstituted…
Tue, Jul 18, 2023
Can I use the kit ADI-EKS-895 to measure HSP90a activity?
ADI-EKS-895 is used to measure the total amount of human HSP90a in a given sample. It cannot be used for…
Tue, Jul 18, 2023
How were the <i>E.coli</i> host cell protein standards generated?
Most of the E.coli strains used in the industry and in the production of biologics are derived from K12. The…
Tue, Jul 18, 2023