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Protein Analysis
What storage conditions are needed for an antibody?
Antibodies have a compact and stable secondary structure making them relatively stable proteins. Specific storage recommendations are listed on the…
Tue, Jul 18, 2023
Do antibodies have an expiration date?
Please check the datasheet for an expiry date. If nothing is mentioned, then most ELS products, stored under the recommended…
Tue, Jul 18, 2023
Why are my ODs different than those shown in the instruction manual?
It is normal to see a small variation in OD values between experiments. OD values can be affected by small…
Tue, Jul 18, 2023
What is the best way to analyze my data? What is 4-PL curve-fitting?
For the most accurate results we recommend that 4 parameter logistic (4-PL) curve fitting software be used. The object of…
Tue, Jul 18, 2023
Competitive Immunoassays
In competitive ELISA Assays, the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a…
Tue, Jul 18, 2023
What kind of instrumentation is required to read a CLIA assay?
A plate luminometer is necessary to read the RLU (relative light units) generated by these assays. It is not necessary…
Tue, Jul 18, 2023
Immunoassay Basics
Understanding the basic principles of immunoassays is easy. The essential components of antibody-based immunoassay systems are threefold: an antigen that…
Tue, Jul 18, 2023
Immunometric Assays
Immunometric assays, also known as sandwich assays, use two antibodies specific to the antigen to capture or “sandwich” antigen in…
Tue, Jul 18, 2023
Do I have to use a wavelength correction when reading an ELISA plate?
Reading at dual wavelengths corrects for the optical density contributed by the plastic the plate wells are made of as…
Tue, Jul 18, 2023
Can I use a spectrophotometer to read my assay?
No, not unless the product specific instruction manual specifically gives you this option. A microtiter plate reader capable of detecting…
Tue, Jul 18, 2023