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Protein Analysis
Do antibodies have an expiration date?
Please check the datasheet for an expiry date. If nothing is mentioned, then most ELS products, stored under the recommended…
Tue, Jul 18, 2023
Indirect Immunoassays
The enzyme-linked immunosorbent assay (ELISA Kits), also known as an indirect immunoassay, is a third type of immunoassay commonly used…
Tue, Jul 18, 2023
When is it appropriate to use a CLIA instead of an EIA?
A CLIA, or chemiluminescent immunoassay, is more sensitive than an EIA (enzyme immunoassay) and is used when samples contain very…
Tue, Jul 18, 2023
Why are my ODs different than those shown in the instruction manual?
It is normal to see a small variation in OD values between experiments. OD values can be affected by small…
Tue, Jul 18, 2023
What is the best way to analyze my data? What is 4-PL curve-fitting?
For the most accurate results we recommend that 4 parameter logistic (4-PL) curve fitting software be used. The object of…
Tue, Jul 18, 2023
How do I know if I need to dilute or extract my samples?
If your samples contain analyte levels within the dynamic range of the assay at the minimum recommended dilution for your…
Tue, Jul 18, 2023
What species does your kit work with?
Kits where the molecule is conserved among all species (e.g. eicosanoids, steroids) are species independent, and may be used with…
Tue, Jul 18, 2023
Can I use a spectrophotometer to read my assay?
No, not unless the product specific instruction manual specifically gives you this option. A microtiter plate reader capable of detecting…
Tue, Jul 18, 2023
Do I have to use a wavelength correction when reading an ELISA plate?
Reading at dual wavelengths corrects for the optical density contributed by the plastic the plate wells are made of as…
Tue, Jul 18, 2023
What is the minimum recommended dilution for samples? Can I dilute less?
This is the minimum dilution required to eliminate matrix interference in the assay. In general, this data is collected using…
Tue, Jul 18, 2023