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What is the recommended assay concentration range for KillerTRAIL™ and SuperKillerTRAIL™?
The recommended working dilution range for KillerTRAIL™ is 10-100 ng/ml, and 2-100 ng/ml for SuperKillerTRAIL™. However, adjustment may be necessary for the end-user’s specific…
Tue, Jul 18, 2023
Is this Oxytocin ELISA kit species-independent?
Although novel forms of oxytocin with a single amino acid substitution, which may affect overall antibody binding affinity, have been…
Tue, Jul 18, 2023
I am working with rabbit sera. Can I use your Oxytocin ELISA kit for detecting oxytocin in this sample?
Problems may arise when working with rabbit sera because the kit relies on the use of an anti-rabbit IgG. This…
Tue, Jul 18, 2023
Do oxytocin kits work on other sample types other than culture supernatants, milk, plasma or serum?
Cited sample types include cerebral spinal fluids (CSF), feces, plasma, saliva, serum, tissue, and urine.
Tue, Jul 18, 2023
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions for the Oxytocin ELISA kit?
The amount of sample required and the dilutions needed for the assay depend upon the level of oxytocin in the…
Tue, Jul 18, 2023
What are the differences between TRAIL, KillerTRAIL™ and SuperKillerTRAIL™?
To induce apoptosis, TRAIL needs to form multimers, showing increasing activity with increasing multimerization levels. Multimerization of TRAIL can be…
Tue, Jul 18, 2023
Does the Oxytocin ELISA kit work with saliva samples?
Although saliva was not a sample type tested during the development of this kit, there has been significant interest in…
Tue, Jul 18, 2023
Is extraction of oxytocin necessary prior to using the Oxytcin ELISA kit?
Oxytocin ELISA kit does require extraction from serum and plasma. This extraction is required because of matrix interference with serum…
Tue, Jul 18, 2023
Is there an alternative protocol for extraction of oxytocin in serum or plasma samples prior to using the Oxytocin ELISA kit?
The procedure described below is an alternative protocol for extraction of oxytocin from aqueous solutions. 1. Add two volumes of ice…
Tue, Jul 18, 2023