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When using an IMMUNOSET® ELISA development set, how long can I store the components after I reconstitute them?
The reconstituted reagents should be aliquotted and stored at the temperature recommended in the instruction manual for up to three…
Tue, Jul 18, 2023
Can I measure tissue NO with the Nitric Oxide detection kits?
Yes. Samples can be homogenized in the provided Reaction Buffer and then centrifuged. The supernatant should then be filtered through…
Tue, Jul 18, 2023
What protocol can be used for drying samples prior to cAMP/cGMP quantification?
Samples can be dried using either vacuum centrifugation (e.g. SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023
Can I measure the levels of cAMP/cGMP in rabbit sera considering that the plate is coated with an anti-rabbit IgG?
The plate provided in these kits is coated with a generic antibody capable of capturing the cAMP- or cGMP-specific antibody.…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will the type of plates I use for coating affect the assay performance?
We recommend using plates made with high binding plastic, similar to Corning plates (2592). The use of different types of…
Tue, Jul 18, 2023
Can I store acetylated samples for cAMP/cGMP quantification at a later time?
No. We recommend that only enough samples needed for the current run be acetylated. Samples are more stable when stored…
Tue, Jul 18, 2023
How many cells should I work with for the quantification of cAMP or cGMP?
The amount of tissue or number of cells to use is entirely dependent on the experimental conditions. It is recommended…
Tue, Jul 18, 2023
How much Assay Buffer is required to reconstitute my serum samples in prior to cAMP/cGMP quantification?
The volume of Assay Buffer needed to reconstitute samples is completely dependent on experimental conditions. We recommend re-suspending 1mL of…
Tue, Jul 18, 2023
Which protein assay is compatible with lysates obtained with HCl treatment?
This question is actually better suited for a company supplying the protein assay you wish to use. Thermo, for example,…
Tue, Jul 18, 2023
Can I use my own lysis buffer for the preparation of my cell lysates prior to cAMP/cGMP quantification?
The kit has been optimized for using HCl to both lyse and dilute your standards and samples while used in…
Tue, Jul 18, 2023