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Is there a requirement for cell lysis with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001
LEADING LIGHT® Wnt Reporter Assay (ENZ-61001) is a cell-based luciferase and does not require lysis and chemiluminescence signals can be…
Tue, Jul 18, 2023
How do we perform QC for E. coli host cell protein ELISA kit, ENZ-KIT127?
Quality control for E. coli host cell protein ELISA kit (ENZ-KIT127) is performed by randomly selecting multiple plates from each…
Tue, Jul 18, 2023
What could be the reason for low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001?
If observing a low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay (ENZ-61001), please consider the following. The…
Tue, Jul 18, 2023
Buffer salts in 10x PBS-T wash buffer in E. coli host cell protein ELISA kit, ENZ-KIT127 has precipitated upon storage at 4°C. Is this usual and how to dissolve the salts?
On occasions, buffer salts precipitate out of solution at 4°C. Warming the bottle containing buffer at 37°C and frequent vortexing would…
Tue, Jul 18, 2023
Can CHO host cell protein standards (ENZ-PRT122) be used as internal control in Western Blot or as standards in CHO Host cell protein ELISA kit (ENZ-KIT128)?
CHO host cell protein standards (ENZ-PRT122) can be used in Western blot or in an ELISA. With CHO Host cell…
Tue, Jul 18, 2023
What is the concentration of the PGE2-Alkaline phosphatase conjugate in the PGE2 ELISA kit, ADI-900-001?
Concentration of the PGE2-AP conjugate is proprietary and can't be revealed.
Tue, Jul 18, 2023
What is the suggestion for using part of the ADI-900-057, Protein A ELISA kit then re-using the remainder of the kit at a later date?
In general, we would not advise holding the entire kit at 5-6 hours at ambient temperature if this can be…
Tue, Jul 18, 2023
What volume of recombinant PKC or cell extracts should be used with ADI-EKS-420A?
In experiments with mouse brain lysates using the PKC kinase activity kit (ADI-EKS-420A), we found that 0.02 – 2µg of…
Tue, Jul 18, 2023
Can a rough estimate antibody concentration of a-Gal Epitope (Gala1-3Galß1-4GlcNAc-R) monoclonal antibody (M86), ALX-801-090-1 be stated?
The supernatant of ammonium sulfate precipitate hybridoma tissue culture supernatant for The a-Gal epitope monoclonal antibody (ALX-801-090-1) is concentrated and…
Tue, Jul 18, 2023
What is the difference between Hoechst 3342, ENZ-52401 and Hoechst 33258, ENZ-52402?
Hoechst 33258 (ENZ-52402) is less cell permeable than other derivatives, however it also allows for quantitative measurements when plotted in…
Tue, Jul 18, 2023