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Protein signal is saturated.
The concentration from protein sample is too high. Dilute the sample further with 1X Assay Buffer.
Tue, Jul 18, 2023
Values for this assay are reported as pmol/well. How can I convert the values obtained by this kit into a value that is more commonly reported in the literature, such as pmol/L (pM)?
Since the volume of sample added to each well is 50µL, results can be converted to molarity by multiplying by…
Tue, Jul 18, 2023
After having measured the total glutathione content, is it possible to use the same samples on the plate so as to continue the procedure and measure the oxidized form of glutathione?
The measurements of total and oxidized glutathione are two completely different events; it is therefore not possible to use the…
Tue, Jul 18, 2023
Organelles are not sufficiently stained.
Very low concentration of dye was used, or dye was incubated with the cells for an insufficient length of time.…
Tue, Jul 18, 2023
Can whole blood samples be used?
High background may result from the presence of hemoglobin in your sample. Hemoglobin (Hb) has a significant absorption at 340…
Tue, Jul 18, 2023
Nuclear counterstain is too bright compared to the organelle stain.
Different microscopes, cameras and filters may make some signals appear very bright. Reduce the concentration of the nuclear counterstain or…
Tue, Jul 18, 2023
What species can the eicosanoid ELISA kits be used for?
Eicosanoids are not species-specific. However, the antisera used in a particular assay may present an interference effect due to the…
Tue, Jul 18, 2023
When using an eicosanoid ELISA kit, how much indomethacin should I use in my samples?
You should follow the suggestion in the product specific manual, and add up to 10µg per ml of liquid sample.…
Tue, Jul 18, 2023
If I have 2N NaOH hydrolyzed samples for an 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> determination, do I need to extract these samples?
No, the Direct 8-iso-PGF2a kit is specifically designed to help you obtain results as quickly and easily as possible. The…
Tue, Jul 18, 2023
What is the difference between the 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit and the Direct 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit?
The regular 8-iso-PGF2a kit is designed to measure free isoprostane in samples. Direct 8-iso-PGF2a measures total isoprostane levels, both free…
Tue, Jul 18, 2023