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Is the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, specific for live cell imaging? Can cells be fixed after staining?
Yes, the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, is validated on living cells only and tt would not be possible…
Tue, Jul 18, 2023
How is the SA-beta-galactosidase measured with your cellular senescence activity assay (ENZ-KIT129)?
This assay uses a fluorometric substrate to detect SA-b-galactosidase activity in cell lysates. The buffers included in this kit maintain…
Tue, Jul 18, 2023
How many cells should I use with :your Cellular senescence activity assay?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Under what conditions were the cells subjected to create hypoxic conditions with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042? Were the cells grown under 95% N2 5 % CO2 ?
When validating the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, cells were grown in 95% nitrogen and also validated with either…
Tue, Jul 18, 2023
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Can tissue be used with your cellular senescence activity assay (ENZ-KIT129)?
Tissues can be used with this assay. They will need to be re-suspended in the 1X cell lysis buffer provided…
Tue, Jul 18, 2023
Are collagen-coated plates compatible with your cellular senescence activity assay (ENZ-KIT129)?
Collagen will not interfere with this assay. You can, therefore, grow your cells on collagen-coated plates.
Tue, Jul 18, 2023
Why does the cell pre-treatment solution in ENZ-KIT130 appear to be toxic to my cells?
The cell pre-treatment solution is non-toxic to cells and should not induce cell death. However, some cell types do experience…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay(ENZ-KIT130), how long does the fluorescence last?
The fluorescence will last for several hours to one day.
Tue, Jul 18, 2023
How long can the cells be saved until analysis by flow cytometry when using the Cellular senescence live cell analysis assay (ENZ-KIT130)?
Analyzing the results within a few hours is recommended because this assay uses live cells. If the cells lyse, the dye…
Tue, Jul 18, 2023