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Why are Aqua 431 dUTP-labeled probes much harder to detect?
There are two aspects making Aqua-labeled probes harder to detect: First, the aqua fluorophore (DEAC – 7-Diethylaminocoumarin-3-carboxylic acid) has a…
Tue, Jul 18, 2023
Why should post-hybridization washes be carried out?
Post-hybridization washing after ISH will reduce non-specific signal by removing probes that are lightly bound to off-target sequences. Most frequently,…
Tue, Jul 18, 2023
How can slides be stored after ISH?
That depends mostly on fixation/embedding method and the type of ISH performed. After chromogenic ISH on FFPE or cryopreserved tissue,…
Tue, Jul 18, 2023
Can multiple targets be detected with ISH?
Yes, it is common practice to stain with multiple ISH probes in parallel. However, while continuously-labeled immunofluorescent probes can be…
Tue, Jul 18, 2023
Probe Design and Generation – How are DNA probes prepared?
First choice is to explore if commercially ready-to-use probes are available for specific targets. Enzo Life Sciences offers a selection…
Tue, Jul 18, 2023
What is the recommended thickness of the section to perform ISH?
Ideal section thickness depends on fixation and embedding method, as well as microscope that is available. FFPE tissue is ideally…
Tue, Jul 18, 2023
Is there a specifc type of glass slide recommended for ISH?
Positively charged glass slides have proven optimal to draw tissue sample sections for subsequent ISH on. Alternatively, poly-L-lysine or silanized…
Tue, Jul 18, 2023
Guidelines For Handling Small Molecules
These guidelines are general tips for use with handling small molecules. Small molecules require careful handling procedures to preserve their…
Tue, Jul 18, 2023
Guidelines For Handling Proteins
These guidelines are general tips for use with handling proteins. Proteins require careful handling procedures to preserve protein function, stability,…
Tue, Jul 18, 2023
Guidelines for For Handling Antibodies
Antibodies require careful handling procedures to preserve antibody function, stability, and integrity. Please refer to the data sheet(s), product webpage,…
Tue, Jul 18, 2023