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What is the mechanism of the Golgi-ID™ Green assay kit
The Green detection reagent included in the kit ENZ-51028 is a fluorescent sphingolipid analog that is metabolized by cells and…
Tue, Jul 18, 2023
What is the difference between PROTEOSTAT® Aggresome detection kit (ENZ-51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023)?
Both our PROTEOSTAT® Aggresome detection kit (ENZ51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023) rely on the properties of our PROTEOSTAT®…
Tue, Jul 18, 2023
Can ISH be multiplexed with immunostaining?
Yes, both CISH can be combined with immunohistochemistry and FISH can be combined with immunofluorescence. However, multiplexing ISH with immunostaining…
Tue, Jul 18, 2023
The chromogens are dissolving during dehydration, what may be the cause?
While most common chromogens are relatively resistant to organic solvents, some chromogens might be dissolved in xylene. Using xylene substitutes…
Tue, Jul 18, 2023
What are the correction factors for measuring dye incorporation in a NanoDrop?
The correction factors for 260/280nm detection are as follows: Correction Factor at Aqua 431 Green 496 Green 500 Gold 525 Gold 500 Orange…
Tue, Jul 18, 2023
Can the signal of the fluorescent dUTPs be amplified?
Yes. One possibility to amplify the fluorescent signal would be with antibodies against the fluorophore. For example, the Green 496…
Tue, Jul 18, 2023
Can BIO-PROBE®, PATHO-GENE®, or AMPIVIEW® ready-to-use ISH probes be used for FISH?
Biotin-labeled in situ probes are most commonly used for chromogenic detection, they can easily be adapted to fluorescent detection with…
Tue, Jul 18, 2023
Which dUTPs are best combined for multiplexing?
If probes are selected so that both location and color contribute to identification, it is theoretically possible to combine all…
Tue, Jul 18, 2023
Can the dUTPs be used for TUNEL assays or only for ISH probe labeling?
The labeled dUTPs can be used for a variety of applications besides in situ hybridization. They can be used as…
Tue, Jul 18, 2023
What is the recommended ratio of dTTP to fluorophore-dUTP when using the Nick translation DNA labeling system 2.0?
The SEEBRIGHT® fluorophore-labeled dUTPs are supplied as a 1 mM solution. We recommend to prepare working concentration of 0.3 mM…
Tue, Jul 18, 2023