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What is the recommendation to reconstitute the SEEBRIGHT® lyophilized dUTPs, ENZ-42853L, ENZ-42843L, ENZ-42831L, ENZ-42842L?
Dissolving in 10 mM TE pH8, is the preferred method since the nucleotides are stable for long term -20°C storage.…
Tue, Jul 18, 2023
What is the suggested volume of SEEBRIGHT® Red 598 dUTP, ENZ-42855 and SEEBRIGHT® Green 496 dUTP, ENZ-42831 with Nick translation DNA labeling system 2.0, ENZ-GEN111-0050?
We suggest to reconstitute the dyes (0.3 mM) and use the same volumes as mentioned in the manual of the…
Tue, Jul 18, 2023
Can you provide an overview of steps in Intracellular Calcium mobilization assay with FLUOFORTE® Calcium assay kit, ENZ-51016 ?
The main steps for the FLUOFORTE® Calcium assay kit (ENZ51016) involves loading cells with dye that fluoresce when exposed to…
Tue, Jul 18, 2023
How can staining be improved with the PROTEOSTAT® Aggresome detection kit, ENZ-51035?
Optimize the dilution of the dye provided with PROTEOSTAT® Aggresome detection kit (ENZ-51035) to improve staining. Also incubationb time can…
Tue, Jul 18, 2023
How can photobleaching be minimized with PROTEOSTAT® Aggresome detection kit, ENZ-51035?
PROTEOSTAT® dye is light sensitive. Avoid direct exposure of the reagent to intense light. In a microscopy experiment with PROTEOSTAT®…
Tue, Jul 18, 2023
Is there a requirement for bridging when changing lots of E. coli host cell protein ELISA kit, ENZ-KIT127?
A bridging study is required if this is a part of defined or specified SOP for a facility or a…
Tue, Jul 18, 2023
How are Quality control tests for each lot of E.coli host cell protein ELISA kit, ENZ-KIT127 performed?
Quality control testing is performed by randomly selecting multiple plates from each lot. All lot-specific components are tested together using…
Tue, Jul 18, 2023
How long can anti-DNP biotin antibody and streptavidin-HRP be stored once reconstituted for use with Protein Carbonyl ELISA, ALX-850-312?
Anti-DNP-biotin antibody (G) can be stored at -20 °C, for 1-2 weeks and streptavidin-horseradish peroxidase (H) can also be stored…
Tue, Jul 18, 2023
Can samples, controls and standards be stored once they are DNP derivatized Protein Carbonyl ELISA kit, ALX-850-312?
Storing samples, controls or standards after derivatization is not recommended. So, careful planning of work flow is needed to complete…
Tue, Jul 18, 2023
Can Standards and Samples be derivatized on different days with Protein Carbonyl ELISA kit, ALX-850-312?
Standards, Controls and samples should be treated simultaneously with DNP on the same day in parallel to maintain consistency. When…
Tue, Jul 18, 2023