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Organelle Detection
Can antifade mounting medium hardset (ENZ-53002, ENZ-53003) be used with fluorescein and rhodamine?
Yes, the antifade mounting medium hardeset (ENZ-53002, ENZ-53003) is recommended for use on fiwed cells and tissus that have been…
Tue, Jul 18, 2023
What is the application of the antifade mounting medium hardset (ENZ-53002, ENZ-53003) ?
The Antifade mounting medium hardeset can be used as a mouning medium fo fixed cells and tissues, stained with fluorescent…
Tue, Jul 18, 2023
Does the appearance of green aggregates under the microscope suggest increased mitochondrial potential with Mito-ID® Membrane potential detection kit, ENZ-51018?
The MITO-ID® dye in the MITO-ID® Membrane potential detection kit (ENZ-51018) is a dual-emission cationic dye to measure the mitochondrial…
Tue, Jul 18, 2023
How long does the signal last with Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019?
Time-course study of mitochondrial membrane potential changes with the Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019 can be…
Tue, Jul 18, 2023
What is the recommendation for dye to cell ratio for microscopy and flow cytometry staining with the MITO-ID® Membrane potential detection kit, ENZ-51018?
For detailed information on the protocol, please follow the indications provided in the manual avalable from download on MITO-ID® Membrane…
Tue, Jul 18, 2023
Following staining with Mito-ID® Red detection kit, ENZ-51007, are the cells viable and is it possible to propagate cells?
The MITO-ID® Membrane potential dye (ENZ-51007) is not toxic to the cells at the concentrations and staining time recommended. Experiments demonstrated…
Tue, Jul 18, 2023
Can Mito-ID® Red detection kit (GFP-Certified®), ENZ-51007 and Mito-ID® Red antifade reagent, ENZ-53002 stain yeast cells ?
Staining of yeast cells with Mito-ID® Red detection kit has not been tested. The kit could work in yeast cells with optimization…
Tue, Jul 18, 2023
What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023
What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?
The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.
Tue, Jul 18, 2023
What could be the reason when no red fluorescence is detected in a sample containing both live and dead cells if the blue DAPI works well and the protocols were followed as recommended?
The filter settings should be checked to determine that they are optimal. Since the blue signal is stronger than the…
Tue, Jul 18, 2023