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Organelle Detection
Can cells be fixed and permeabilized after staining with Nuclear-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006)?
Cells can be fixed and permeabilized after staining with the NUCLEAR-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006). For best results,…
Tue, Jul 18, 2023
What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023
What is the chemical identity of the LYSO-ID® dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
The precise chemical identity of the LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 is proprietary. These are a cell…
Tue, Jul 18, 2023
What is the mechanism of action for LYSO-ID® detection dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 are proprietary cationic amphiphilic tracers (CAT) that rapidly and selectively stains acidic…
Tue, Jul 18, 2023
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) appears to be clumped. What is the recommendation to get this in liquid form?
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) needs to be warmed at 37-42°C for about 20 minutes, then vortexedt, if clumps are…
Tue, Jul 18, 2023
What is the application of the antifade mounting medium hardset (ENZ-53002, ENZ-53003) ?
The Antifade mounting medium hardeset can be used as a mouning medium fo fixed cells and tissues, stained with fluorescent…
Tue, Jul 18, 2023
Does the appearance of green aggregates under the microscope suggest increased mitochondrial potential with Mito-ID® Membrane potential detection kit, ENZ-51018?
The MITO-ID® dye in the MITO-ID® Membrane potential detection kit (ENZ-51018) is a dual-emission cationic dye to measure the mitochondrial…
Tue, Jul 18, 2023
Can Mito-ID® Red detection kit (GFP-Certified®), ENZ-51007 and Mito-ID® Red antifade reagent, ENZ-53002 stain yeast cells ?
Staining of yeast cells with Mito-ID® Red detection kit has not been tested. The kit could work in yeast cells with optimization…
Tue, Jul 18, 2023
What could be the reason when no red fluorescence is detected in a sample containing both live and dead cells if the blue DAPI works well and the protocols were followed as recommended?
The filter settings should be checked to determine that they are optimal. Since the blue signal is stronger than the…
Tue, Jul 18, 2023
How long is the NUCLEAR-ID® red dye (ENZ-52406) retained by the cells and is the dye toxic to the cells?
NUCLEAR-ID® red DNA stain ( ENZ-52406) is fairly stable. We do not have specifics on how long the dye would be…
Tue, Jul 18, 2023