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Organelle Detection
Following staining with Mito-ID® Red detection kit, ENZ-51007, are the cells viable and is it possible to propagate cells?
The MITO-ID® Membrane potential dye (ENZ-51007) is not toxic to the cells at the concentrations and staining time recommended. Experiments demonstrated…
Tue, Jul 18, 2023
Can Mito-ID® Red detection kit (GFP-Certified®), ENZ-51007 and Mito-ID® Red antifade reagent, ENZ-53002 stain yeast cells ?
Staining of yeast cells with Mito-ID® Red detection kit has not been tested. The kit could work in yeast cells with optimization…
Tue, Jul 18, 2023
Can antifade mounting medium hardset (ENZ-53002, ENZ-53003) be used with fluorescein and rhodamine?
Yes, the antifade mounting medium hardeset (ENZ-53002, ENZ-53003) is recommended for use on fiwed cells and tissus that have been…
Tue, Jul 18, 2023
What is the application of the antifade mounting medium hardset (ENZ-53002, ENZ-53003) ?
The Antifade mounting medium hardeset can be used as a mouning medium fo fixed cells and tissues, stained with fluorescent…
Tue, Jul 18, 2023
Can cells be fixed and permeabilized after staining with Nuclear-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006)?
Cells can be fixed and permeabilized after staining with the NUCLEAR-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006). For best results,…
Tue, Jul 18, 2023
What is the mechanism of action for LYSO-ID® detection dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 are proprietary cationic amphiphilic tracers (CAT) that rapidly and selectively stains acidic…
Tue, Jul 18, 2023
What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) appears to be clumped. What is the recommendation to get this in liquid form?
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) needs to be warmed at 37-42°C for about 20 minutes, then vortexedt, if clumps are…
Tue, Jul 18, 2023
How specific is the NUCLEAR-ID® red dye (ENZ-52406) for dsDNA? What is the binding capacity of the dye to ssRNA, ssDNA and DNA:RNA Hybrids?
The dye stains dsDNA. Data is not available for binding capacity to ssRNA, ssDNA and DNA:RNA hybrids.
Tue, Jul 18, 2023
What could be the reason when no red fluorescence is detected in a sample containing both live and dead cells if the blue DAPI works well and the protocols were followed as recommended?
The filter settings should be checked to determine that they are optimal. Since the blue signal is stronger than the…
Tue, Jul 18, 2023