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Home Life Sciences Life Sciences Products Cellular Analysis CELLESTIAL® Assays Organelle Detection Page 2

Organelle Detection

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Antifade mounting medium hardset (ENZ-53002, ENZ-53003) appears to be clumped. What is the recommendation to get this in liquid form?

Antifade mounting medium hardset (ENZ-53002, ENZ-53003) needs to be warmed at 37-42°C for about 20 minutes, then vortexedt, if clumps are…

Tue, Jul 18, 2023

Organelle Detection
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Can antifade mounting medium hardset (ENZ-53002, ENZ-53003) be used with fluorescein and rhodamine?

Yes, the antifade mounting medium hardeset (ENZ-53002, ENZ-53003) is recommended for use on fiwed cells and tissus that have been…

Tue, Jul 18, 2023

Organelle Detection
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What is the application of the antifade mounting medium hardset (ENZ-53002, ENZ-53003) ?

The Antifade mounting medium hardeset can be used as a mouning medium fo fixed cells and tissues, stained with fluorescent…

Tue, Jul 18, 2023

Organelle Detection
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Does the appearance of green aggregates under the microscope suggest increased mitochondrial potential with Mito-ID® Membrane potential detection kit, ENZ-51018?

The MITO-ID® dye in the MITO-ID® Membrane potential detection kit (ENZ-51018) is a dual-emission cationic dye to measure the mitochondrial…

Tue, Jul 18, 2023

Organelle Detection
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How long does the signal last with Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019?

Time-course study of mitochondrial membrane potential changes with the Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019 can be…

Tue, Jul 18, 2023

Organelle Detection
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What is the recommendation for dye to cell ratio for microscopy and flow cytometry staining with the MITO-ID® Membrane potential detection kit, ENZ-51018?

For detailed information on the protocol, please follow the indications provided in the manual avalable from download on MITO-ID® Membrane…

Tue, Jul 18, 2023

Organelle Detection
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Following staining with Mito-ID® Red detection kit, ENZ-51007, are the cells viable and is it possible to propagate cells?

The MITO-ID® Membrane potential dye (ENZ-51007) is not toxic to the cells at the concentrations and staining time recommended. Experiments demonstrated…

Tue, Jul 18, 2023

Organelle Detection
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Can Mito-ID® Red detection kit (GFP-Certified®), ENZ-51007 and Mito-ID® Red antifade reagent, ENZ-53002 stain yeast cells ?

Staining of yeast cells with Mito-ID® Red detection kit has not been tested.  The kit could work in yeast cells with optimization…

Tue, Jul 18, 2023

Organelle Detection
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Is the Red NUCLEAR-ID® dye in the NUCLEAR-ID® Blue/Red cell viability reagent provided with reference ENZ-53005 the same dye as NUCLEAR-ID Red for cell cycle analysis provided with reference ENZ-51008?

No, these are two different dyes.

Tue, Jul 18, 2023

Organelle Detection
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What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?

The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.

Tue, Jul 18, 2023

Organelle Detection
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