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High fluorescence signal observed in negative control
Sample contains interfering substances. The assay is compatible with commonly used buffers (PBS, Tris, HEPES) and excipients (trehalose and sucrose),…
Tue, Jul 18, 2023
Protein signal is saturated.
The concentration from protein sample is too high. Dilute the sample further with 1X Assay Buffer.
Tue, Jul 18, 2023
Poor staining is observed in the positive control.
Stained reagents have been exposed to strong light. Protect samples from exposure to strong light and analyze them immediately after…
Tue, Jul 18, 2023
The dyes fail to stain acidic organelles in fixed and/or permeabilized cells.
The following kits are only suitable for live cell staining only. Use them only for live cell analysis. Cyto-ID® Autophagy…
Tue, Jul 18, 2023
Nuclear counterstain is too bright compared to the organelle stain.
Different microscopes, cameras and filters may make some signals appear very bright. Reduce the concentration of the nuclear counterstain or…
Tue, Jul 18, 2023
Organelles are not sufficiently stained.
Very low concentration of dye was used, or dye was incubated with the cells for an insufficient length of time.…
Tue, Jul 18, 2023
Glossary
Antibody: An immunoglobulin that recognizes some portion of an antigen molecule. The antibody contains a species-specific Fc region and antigen-specific…
Tue, Jul 18, 2023
Can whole blood samples be used?
High background may result from the presence of hemoglobin in your sample. Hemoglobin (Hb) has a significant absorption at 340…
Tue, Jul 18, 2023
What can interfere with the assay?
Reducing agents (such as ascorbic acid, beta-mercaptoethanol, dithiothreitol, and cysteine) and thiol reactive compounds (such as maleimides) are known to…
Tue, Jul 18, 2023
Values for this assay are reported as pmol/well. How can I convert the values obtained by this kit into a value that is more commonly reported in the literature, such as pmol/L (pM)?
Since the volume of sample added to each well is 50µL, results can be converted to molarity by multiplying by…
Tue, Jul 18, 2023