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Life Sciences
In the sPLA2 activity kit (ADI-907-002), how is a Unit defined?
One unit of phospholipase activity is the amount of enzyme needed to hydrolyze 1.0 µmole of L-a-phosphatidylcholine to L-a-lysophosphatidylcholine and…
Tue, Jul 18, 2023
Immunoassay Kits Glossary
Alkaline phosphatase: (1) a phosphatase enzyme found in high concentrations in bone, liver, kidneys, and placenta, is active in an…
Tue, Jul 18, 2023
High fluorescence signal observed in negative control
Sample contains interfering substances. The assay is compatible with commonly used buffers (PBS, Tris, HEPES) and excipients (trehalose and sucrose),…
Tue, Jul 18, 2023
Poor signal observed in the assay
Staining reagent has been exposed to strong light. Protect reagents from exposure to strong light.
Tue, Jul 18, 2023
More than one aggregation peak is observed.
A protein might have multiple domains that start aggregation at different temperatures. For most samples, any aggregation is an indication…
Tue, Jul 18, 2023
Inconsistent results between PDI assay experiments.
This is caused by inappropriate Stop Reagent addition. Be sure to pre-incubate with Stop Reagent to terminate both the enzyme…
Tue, Jul 18, 2023
Low or no fluorescent signal in positive control
Species of interest may react with each other, thus attenuating the expected signal. ROS is a complex interacting system. Blocking…
Tue, Jul 18, 2023
Can I use the COX activity kit to distinguish between COX-1 and -2?
The COX Activity kits measure activity alone, distinguishing between COX-1 or -2. In order to differentiate between the two enzymes,…
Tue, Jul 18, 2023
Can I use cell culture samples in the COX Activity assay?
Cell culture samples are compatible with this kit. Since this is an activity assay rather than a quantitative immunoassay, there…
Tue, Jul 18, 2023
Do I need a luminometer with double injectors for this assay?
Yes. A single injector will not provide good results. You cannot pre-mix the arachidonic acid and substrate to make a…
Tue, Jul 18, 2023