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Life Sciences
Do you check for cross-reactivity with other steroids?
Yes, cross-reactivities for a number of related steroid compounds were determined and are listed in the kit insert.
Tue, Jul 18, 2023
Do you sell positive controls that can be run with your steroid ELISA kits?
We do not provide positive control samples with our kits. If you need to ensure accurate recovery of any analyte…
Tue, Jul 18, 2023
How can I overcome sample matrix interference?
The easiest way to overcome sample matrix interference is by diluting your sample with assay buffer. In order to determine…
Tue, Jul 18, 2023
How should I test for possible matrix interference with my samples?
Matrix interference describes the situation where the sample matrix (buffer salts, pH, additives like detergents or solvents, proteins, …) interferes…
Tue, Jul 18, 2023
Which is the best dilution for my sample type?
In the manual, section “Sample recoveries”, we provide a list of recommended dilutions. Those are the dilutions our development scientists…
Tue, Jul 18, 2023
Why is there sample dilution necessary in this ELISA Kit?
There are several reasons for dilutions. In some cases samples might read above the standard curve, thus requiring a dilution…
Tue, Jul 18, 2023
How does the steroid displacement reagent (SDR) work?
Steroids in circulation are almost all ionically bound to carrier proteins such as albumin or more specific binding proteins. Treating…
Tue, Jul 18, 2023
What does ProteoStat® dye bind to and what is the mechanism of detection of protein aggregation?
The molecular rotor dye is non-fluorescent due to free rotation around a central carbon-carbon single bond separating different aromatic portions of…
Tue, Jul 18, 2023
Are there any limitations regarding particle size of the aggregates that can be detected with the ProteoStat® dye?
The ProteoStat® dye detects in the subvisible to visible range from ~ 50 µm to 1 mm.
Tue, Jul 18, 2023
What detergents are compatible with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027 and ProteoStat® Protein Aggregation Assay?
The ProteoStat® dye is compatible with some detergents, such as 0.1% Tween 20, but this varies with the protein used. …
Tue, Jul 18, 2023