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Life Sciences
The kit insert indicates that the PKA enzyme is provided as positive control. Is it possible to use it to perform a standard curve?
The active PKA kinase provided in the kit is to be used as a positive control and is not intended…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
How should serum samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood in a covered test tube. If commercially available tubes are to be used, the researcher should use…
Tue, Jul 18, 2023
Will my assay results vary depending on the sample type I am going to analyze with the NGAL (human) ELISA kit?
Analyses of samples of serum, EDTA plasma, citrate plasma, heparin plasma or urine showed no significant differences in analytical recovery,…
Tue, Jul 18, 2023
How long can I use an ELISA kit after expiry?
Once ELISA kits are expired their performance can no longer be guaranteed. However, ELISA kits do not typically stop working entirely on their…
Tue, Jul 18, 2023
It appears that the positive control is not working properly. What could be the reason?
The nature of that protein is that it can quickly degrade unless it is handled very carefully. It must be…
Tue, Jul 18, 2023
How can the high background be reduced?
During incubation with NBT/BCIP Reaction Mixture, localized blue-purple precipitate is formed. Decreasing the incubation time with NBT/BCIP Reaction Mixture can reduce the…
Tue, Jul 18, 2023
Can I use the Testosterone ELISA kit with human plasma and serum samples?
Sample types that have been validated are indicated on our website in the field “Application” and in the kit insert.…
Tue, Jul 18, 2023
Can the Progesterone ELISA kit be used with samples from any species?
Progesterone is conserved across all species and the antibody provided in the kit will bind to progesterone independently from the…
Tue, Jul 18, 2023
How should I treat samples with the steroid displacement reagent (SDR) for analysis with the Progesterone ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: Add 1µl of SDR per…
Tue, Jul 18, 2023