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Life Sciences
Can Mito-ID® Red detection kit (GFP-Certified®), ENZ-51007 and Mito-ID® Red antifade reagent, ENZ-53002 stain yeast cells ?
Staining of yeast cells with Mito-ID® Red detection kit has not been tested. The kit could work in yeast cells with optimization…
Tue, Jul 18, 2023
MSDS – Safety Data Sheets
All our SDSs are available for download directly from the product page.On the product page, please click the SDS link…
Tue, Jul 18, 2023
Is BIO-PROBE® 3’-Oligonucleotide labeling kit with bio-16-ddUTP, ENZ-42730-31 compatible with EMSA?
BIOPROBE® 3′-Oligonucleotide labeling kit with bio-16-ddUTP, ENZ-42730-31can be used for gel shift assays since the reaction yields uniformly labeled product.
Tue, Jul 18, 2023
What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023
What is the reason for the use of dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020)? Should this be used with all cell lines or is there an exception?
The use of the dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020) can help preventing the…
Tue, Jul 18, 2023
How long is the CYTO-ID® long-term cell tracer dye (ENZ-51036, ENZ-51037) retained in the cell and what cells were used to validate the assay?
The dye is well retained by cells for up to 96-120 hours after loading depending on how fast the cells…
Tue, Jul 18, 2023
Can you provide an overview of cell proliferation experiments with the CYTO-ID® long-term cell tracer kits (ENZ-51036, ENZ-51037)?
It is possible to kinetically monitor the ability of the cells to proliferate with CYTO-ID® Red long-term cell tracer kit…
Tue, Jul 18, 2023
What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?
The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.
Tue, Jul 18, 2023
What is the structure of the FluoForte dye and how does the dye detect Calcium levels in the cell?
The fluorogenic calcium-binding dye is a proprietary acetoxymethyl (AM) ester, which is cell-permeable. Once the dye enters the cells, it…
Tue, Jul 18, 2023
Is the dye efflux inhibitor (reagent B) in the FluoForte® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020), Probenecid ? Should this be added to every cell type to prevent the FluoForte from leaking out of the cells?
The inhibitor is similar in properties to Probenecid. Cells lacking active MDR pumps do not require addition of efflux inhibitor. Depending…
Tue, Jul 18, 2023