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Life Sciences
Why do you use protein A affinity columns to purify the antibodies included in ENZ-KIT127, ENZ-KIT128, and ENZ-KIT162 instead of HCP affinity columns?
The main reason is product consistency. Protein A affinity columns have been around for quite a while. They are well…
Tue, Jul 18, 2023
I have different cell lysates which I would like to run with ADI-EKS-895, HSP90a (human) ELISA kit, but I am not sure if the formulation of my lysis buffer is identical to the extraction buffer present in the kit. Will I be able to analyze my samples
The exact formulation of our extraction buffer is proprietary and cannot be disclosed. The buffer is optimized to achieve complete…
Tue, Jul 18, 2023
Can the ADI-EKS-895 be used to measure mouse HSP90a?
No, the capture antibody does not react with mouse Hsp90a, which prevents the assay from being able to recognize those…
Tue, Jul 18, 2023
Which type of plasma sample do you recommend for using with ADI-900-175?
The kit was validated with citrate plasma samples. EDTA plasma will likely work fine as long as the final EDTA…
Tue, Jul 18, 2023
How should I store reconstituted standard, antibody and conjugatefor ADI-900-175?
The standard, antibody and conjugate are single use items and should to be stored at -20°C. Any leftover of reconstituted…
Tue, Jul 18, 2023
How were the CHO host cell protein standards generated?
The CHO host cell protein standards were prepared from multiple CHO K1 cell lines growing in suspension and in chemically…
Tue, Jul 18, 2023
Are the polyclonal antibodies against <i>E.coli</i>, CHO, and HEK293T host cell proteins purified and by what method?
These antibodies were indeed purified using MabSelect™ (Protein A affinity column) from GE Healthcare.
Tue, Jul 18, 2023
Does the Hypoxia Red Detection Reagent provided with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, retain the red stain during oxygenation?
The hypoxia dye in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, does not fade during oxygenation.
Tue, Jul 18, 2023
How long does the fluorescence last post-staining with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
Storing the samples stained with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, protected from exposure to direct light would help…
Tue, Jul 18, 2023
Is the peptide substrate coated on the microtiter plate present in the ADI-EKS-390A kit specific for PKA?
No, the peptide substrate in the PKA kinase activity kit (ADI-EKS-390A) is not specific to PKA and therefore it can…
Tue, Jul 18, 2023