Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Genomic Analysis
Enzo suggests using 50 – 100 µg of Cot-1 DNA per sample when hybridizing the DNA labeled with the CGH labeling kits to Agilent arrays, is that correct?
The suggested amount of Cot-1 DNA in the protocols is correct. However, there is no theoretical reason why Enzo's kits…
Tue, Jul 18, 2023
Does genomic DNA need to be fragmented for labeling with Enzo’s CGH labeling kits?
Restriction digestion or fragmentation of the isolated genomic DNA is not required prior to labeling with our CYTAG® CGH (ENZ-42671)…
Tue, Jul 18, 2023
How can the quality of DNA labeling by Enzo’s CGH labeling kits be analyzed?
We recommend using a NanoDrop UV-VIS Spectrophotometer in the microarray measurement mode to determine yield and dye incorporation. Use 1.5…
Tue, Jul 18, 2023
Are Enzo’s CGH labeling kits suitable for single nucleotide polymorphism (SNP) analysis?
Our CYTAG® TotalCGH Labeling Kit (ENZ-42674) is perfectly suited for CGH+SNP array analysis. Our CYTAG® CGH (ENZ-42671) and CYTAG® Super…
Tue, Jul 18, 2023
Which kit would work for labeling DNA fragments for an microarray?
BIOARRAY® Terminal labeling kit with biotin-ddUTP for DNA probe array assays, ENZ-42630 is the gold standard for end-labeling in microarrays…
Tue, Jul 18, 2023
What are the standard QC parameters this kit must accommodate?
For a typical labeling reaction with an input of 500 ng of high quality genomic DNA, the yield should be…
Tue, Jul 18, 2023
Can DNA labeled with the Enzo Life Sciences kit be used on a Roche NimbleGen™ platform?
Yes, it can be used. It has been noted by cytogenetics labs that Enzo’s CGH labeling kit works perfectly well…
Tue, Jul 18, 2023
Bright yellow array with reduced magnitude of expected changes
This can be due to insufficient Cot-1 DNA, blocking agents or hybridization and washing conditions that are not stringent enough.
Tue, Jul 18, 2023
Good DLRs in the CGH analytics QC report, but some oligos show whiskering or significant deviation from the expected log2 ratio
We suggest keeping the Cot-1 DNA at 50 µg regardless of the hybridization volume.
Tue, Jul 18, 2023
Overview
Array comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with…
Tue, Jul 18, 2023