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General Questions
Four-color IHC staining protocol
The protocol described below is a sequential staining process and may be adapted to any desired number of colors by adding…
Tue, Jul 18, 2023
Glossary
Antibody: An immunoglobulin that recognizes some portion of an antigen molecule. The antibody contains a species-specific Fc region and antigen-specific…
Tue, Jul 18, 2023
Intracellular Staining Protocol: Two Step Fix/Perm
Two Step Fixation and Permeabilization Materials: Maintain Reagents and buffers at 4°C. Fluorochrome-conjugated primary antibody Fluorochrome-conjugated isotype control antibody Fixation…
Tue, Jul 18, 2023
Intracellular Staining Protocol: One Step Fix/Perm
One Step Fixation and Permeabilization Materials: Maintain Reagents and buffers at 4°C. Fluorochrome-conjugated primary antibody Fluorochrome-conjugated isotype control antibody Fixation/Permeabilization…
Tue, Jul 18, 2023
Extracellular Staining Protocol
Materials: Maintain Reagents and buffers at 4°C. Fluorochrome-conjugated primary antibody Fluorochrome-conjugated isotype control antibody Fixation Buffer, ADI-950-011 PBS (Phosphate Buffered…
Tue, Jul 18, 2023
Fixation of the Cytoskeleton
Fixation of the cytoskeleton If components of the cytoskeleton are of interest, cells can be extracted prior to fixation. Rinse…
Tue, Jul 18, 2023
Paraformaldehyde/Glutaraldehyde Fixation
Paraformaldehyde/glutaraldehyde fixation(method of choice for double labeling of membrane bound and cytoskeletal antigens) Rinse cells with PBS at room temperature.…
Tue, Jul 18, 2023
Western Blot Protocol for use with GPCR Polyclonal Antibodies from Proteimax
Note: The following protocol has been developed for use with GPCR polyclonal antibodies manufactured by Proteimax. Other antibodies have not…
Tue, Jul 18, 2023
Immunometric ELISA
One of the most useful of the immunoassays is the two antibody immunometric or “sandwich” ELISA. This assay is used…
Tue, Jul 18, 2023
Competitive ELISA Assay
For small molecule analytes, typically less than 10 kDa, the competitive ELISA Kit is used. An advantage of this assay…
Tue, Jul 18, 2023