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ELISA Immunoassays
What does Standard Diluent refer to in several ELISA user manual when cell culture supernatant is used?
Standard diluent refers to either the tissue culture media (a.k.a. non-conditioned media) or the assay buffer provided. So, the supernatant…
Tue, Jul 18, 2023
What are important critical factors for success while working with Protein A ELISA kit, ADI-900-057
Following are two critical factors to be considered: 1. If samples contain IgG, standards must be boiled. 2. Diluted standards…
Tue, Jul 18, 2023
Where do I find validation data for Protein A ELISA kit, ADI-900-057 since this is not listed on datasheet?
Description of Validation experiments and the data for Protein A ELISA kit (ADI-900-057) are listed in pages from 9-11 of…
Tue, Jul 18, 2023
How is the Protein A ELISA kit, ADI-900-057 validated?
The Protein A ELISA Kit (ADI-900-057) recognizes natural and recombinant forms of Protein A. Four Protein A constructs were evaluated…
Tue, Jul 18, 2023
What information does Lot specific Certificate of Analysis for Protein A ELIA kit, ADI-900-057 have?
Lot specific Certificate of Analysis for Protein A ELISA kit (ADI-900-057) lists: assay range, OD values for High, Medium, Low,…
Tue, Jul 18, 2023
Can Sample diluent (80-1504) be used to dilute antibody if one runs out of antibody diluent (80-1759) with HO-1 (human), ELISA kit, ADI-EKS-800?
We do not recommend using Sample diluent (80-1504) to dilute antibody instead of antibody diluent (80-1759) with HO-1 (human), ELISA…
Tue, Jul 18, 2023
Can samples, controls and standards be stored once they are DNP derivatized Protein Carbonyl ELISA kit, ALX-850-312?
Storing samples, controls or standards after derivatization is not recommended. So, careful planning of work flow is needed to complete…
Tue, Jul 18, 2023
Can Standards and Samples be derivatized on different days with Protein Carbonyl ELISA kit, ALX-850-312?
Standards, Controls and samples should be treated simultaneously with DNP on the same day in parallel to maintain consistency. When…
Tue, Jul 18, 2023
Can Excel be used to plot read outs from ELISA? How to calculate reads from ELISA using Excel?
Our recommendation is to analyze ELISA reads by 4P curve fit. Many plate readers do have the ability to perform…
Tue, Jul 18, 2023
Why is 4P curve fit curve recommended for ELISA data analysis and where can I find access to this data analysis software?
For the most accurate results, we normally recommend to use a 4-parameter logistic (4-PL) curve fitting software, as 4-PL fitting…
Tue, Jul 18, 2023