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ELISA Immunoassays
Is extraction of vasopressin necessary prior to using the Vasopressin ELISA kit?
Enzo Vasopressin ELISA kit does require extraction of vasopressin from all sample types for accurate determination.
Tue, Jul 18, 2023
Is this Oxytocin ELISA kit species-independent?
Although novel forms of oxytocin with a single amino acid substitution, which may affect overall antibody binding affinity, have been…
Tue, Jul 18, 2023
I am working with rabbit sera. Can I use your Oxytocin ELISA kit for detecting oxytocin in this sample?
Problems may arise when working with rabbit sera because the kit relies on the use of an anti-rabbit IgG. This…
Tue, Jul 18, 2023
Do oxytocin kits work on other sample types other than culture supernatants, milk, plasma or serum?
Cited sample types include cerebral spinal fluids (CSF), feces, plasma, saliva, serum, tissue, and urine.
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions for the Oxytocin ELISA kit?
The amount of sample required and the dilutions needed for the assay depend upon the level of oxytocin in the…
Tue, Jul 18, 2023
Does the Oxytocin ELISA kit work with saliva samples?
Although saliva was not a sample type tested during the development of this kit, there has been significant interest in…
Tue, Jul 18, 2023
Is extraction of oxytocin necessary prior to using the Oxytcin ELISA kit?
Oxytocin ELISA kit does require extraction from serum and plasma. This extraction is required because of matrix interference with serum…
Tue, Jul 18, 2023
Is there an alternative protocol for extraction of oxytocin in serum or plasma samples prior to using the Oxytocin ELISA kit?
The procedure described below is an alternative protocol for extraction of oxytocin from aqueous solutions. 1. Add two volumes of ice…
Tue, Jul 18, 2023
What type of column can be used for oxytocin extraction?
Solid phase extraction (SPE) can be performed using reverse phase C18 columns containing 200 mg of sorbent and a volume capacity…
Tue, Jul 18, 2023
What protocol can be used for drying samples for analysis with the Oxytocin and Arg8-Vasopressin ELISA kits?
Samples can be dried using either vacuum centrifugation (e.g., SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023