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ELISA Immunoassays
Can I use my own lysis buffer for the preparation of my cell lysates prior to cAMP/cGMP quantification?
The kit has been optimized for using HCl to both lyse and dilute your standards and samples while used in…
Tue, Jul 18, 2023
What if my cells are not sufficiently lyzed for cAMP/cGMP quantification after the addition of 0.1M HCl?
Cell or tissue lysis can be enhanced by adding 0.1-1% Triton X-100 to 0.1M HCl prior to use. When used…
Tue, Jul 18, 2023
How much sample do I need to use for cAMP/cGMP quantification and what are the suggested dilutions?
The amount of sample required and the dilutions needed for the assay depend upon the amount of cAMP/cGMP in the…
Tue, Jul 18, 2023
Can I use the cAMP/cGMP ELISA kits for agonism and antagonism studies?
As long as the experimental design is structured so that the end samples can be incorporated into the assay as…
Tue, Jul 18, 2023
What are the expected concentrations of cAMP/cGMP in my samples?
The amounts of cAMP and cGMP in samples depend on sample type, origin, or even treatment. Unfortunately, this is not…
Tue, Jul 18, 2023
I am not getting consistent results with my cAMP/cGMP quantification in cells. I think that my cells may be permeabilized.
For the most consistent results, we recommend that cells be fully disrupted before using the lysate in an assay. Disruption…
Tue, Jul 18, 2023
My maximum binding for acetylated cAMP/cGMP standards and samples is only about 50%. Why?
This occurs if the standards and samples were acetylated but the B0 standard was not. Your data can still be…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the volume or concentration of antibody for coating?
Altering the volume or concentration of the coating antibody will have an impact on assay performance. Users should validate the…
Tue, Jul 18, 2023
Is the acetylation step really necessary prior to cAMP/cGMP quantification?
Samples should be acetylated if they contain a very low concentration of cAMP or cGMP, or when greater sensitivity is…
Tue, Jul 18, 2023
Can any culture medium be used to measure cAMP/cGMP in culture supernatants with cAMP/cGMP ELISA and cAMP/cGMP Complete ELISA kits?
As indicated in the insert, as long as the standard curve is titrated in the same culture media that the…
Tue, Jul 18, 2023