AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
ELISA Immunoassays
What if my cells are not sufficiently lyzed for cAMP/cGMP quantification after the addition of 0.1M HCl?
Cell or tissue lysis can be enhanced by adding 0.1-1% Triton X-100 to 0.1M HCl prior to use. When used…
Tue, Jul 18, 2023
Can I use my own lysis buffer for the preparation of my cell lysates prior to cAMP/cGMP quantification?
The kit has been optimized for using HCl to both lyse and dilute your standards and samples while used in…
Tue, Jul 18, 2023
Which protein assay is compatible with lysates obtained with HCl treatment?
This question is actually better suited for a company supplying the protein assay you wish to use. Thermo, for example,…
Tue, Jul 18, 2023
Can I store acetylated samples for cAMP/cGMP quantification at a later time?
No. We recommend that only enough samples needed for the current run be acetylated. Samples are more stable when stored…
Tue, Jul 18, 2023
How many cells should I work with for the quantification of cAMP or cGMP?
The amount of tissue or number of cells to use is entirely dependent on the experimental conditions. It is recommended…
Tue, Jul 18, 2023
How much Assay Buffer is required to reconstitute my serum samples in prior to cAMP/cGMP quantification?
The volume of Assay Buffer needed to reconstitute samples is completely dependent on experimental conditions. We recommend re-suspending 1mL of…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will the type of plates I use for coating affect the assay performance?
We recommend using plates made with high binding plastic, similar to Corning plates (2592). The use of different types of…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the volume or concentration of antibody for coating?
Altering the volume or concentration of the coating antibody will have an impact on assay performance. Users should validate the…
Tue, Jul 18, 2023
Is the acetylation step really necessary prior to cAMP/cGMP quantification?
Samples should be acetylated if they contain a very low concentration of cAMP or cGMP, or when greater sensitivity is…
Tue, Jul 18, 2023
Can any culture medium be used to measure cAMP/cGMP in culture supernatants with cAMP/cGMP ELISA and cAMP/cGMP Complete ELISA kits?
As indicated in the insert, as long as the standard curve is titrated in the same culture media that the…
Tue, Jul 18, 2023