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ELISA Immunoassays
Can the Cortisol ELISA kit be used with samples from any species?
Cortisol is conserved across all species and the antibody provided in the kit will bind to cortisol independently from the…
Tue, Jul 18, 2023
How does the steroid displacement reagent (SDR) work?
Steroids in circulation are almost all ionically bound to carrier proteins such as albumin or more specific binding proteins. Treating…
Tue, Jul 18, 2023
Why is there sample dilution necessary in this ELISA Kit?
There are several reasons for dilutions. In some cases samples might read above the standard curve, thus requiring a dilution…
Tue, Jul 18, 2023
Which is the best dilution for my sample type?
In the manual, section “Sample recoveries”, we provide a list of recommended dilutions. Those are the dilutions our development scientists…
Tue, Jul 18, 2023
How can I overcome sample matrix interference?
The easiest way to overcome sample matrix interference is by diluting your sample with assay buffer. In order to determine…
Tue, Jul 18, 2023
Do you sell positive controls that can be run with your steroid ELISA kits?
We do not provide positive control samples with our kits. If you need to ensure accurate recovery of any analyte…
Tue, Jul 18, 2023
How should I test for possible matrix interference with my samples?
Matrix interference describes the situation where the sample matrix (buffer salts, pH, additives like detergents or solvents, proteins, …) interferes…
Tue, Jul 18, 2023
Immunoassay Good Lab Practices
Always read and follow the product instructions. The information contained in the kit manual is your first source of knowledge…
Tue, Jul 18, 2023
Units Conversion Table
Molecule Formula Weight Units Conversion Factor Other Units 2,3-dinor-6-keto-Prostaglandin F1a 342.5 pg/ml 2.92 pmol/l 6-keto-Prostaglandin F1a 370.5 pg/ml 2.7 pmol/l…
Tue, Jul 18, 2023
Immunoassay Kits Glossary
Alkaline phosphatase: (1) a phosphatase enzyme found in high concentrations in bone, liver, kidneys, and placenta, is active in an…
Tue, Jul 18, 2023