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ELISA Immunoassays
Can the Corticosterone ELISA kit be used with samples from any species?
Corticosterone is conserved across all species and the antibody provided in the kit will bind to corticosterone independently from the…
Tue, Jul 18, 2023
I am interested in testing corticosterone levels in feces. What is the recommended way to prepare feces samples for this assay?
The mouse and rhinoceros fecal samples mentioned in the kit insert were collected and processed in collaboration with a zoo.…
Tue, Jul 18, 2023
How should I treat my samples with the steroid displacement reagent (SDR)?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: add 2.5µl of SDR per…
Tue, Jul 18, 2023
Can I use the 17ß-Estradiol ELISA kit for serum and plasma samples?
Sample types that have been validated in our laboratories are culture supernatants and saliva. For serum and plasma samples we…
Tue, Jul 18, 2023
Can the 17ß-Estradiol ELISA kit be used with samples from any species?
17ß-Estradiol is conserved across all species and the antibody provided in the kit will bind to 17ß-Estradiol independently from the…
Tue, Jul 18, 2023
How should I treat my samples with the steroid displacement reagent (SDR) for analysis with the 17ß-Estradiol ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: 1µl of SDR per 99µl…
Tue, Jul 18, 2023
My sample type is not listed in your insert. Can the kit be used anyway?
All sample types that have been positively tested are indicated on our website in the field “Application” and in the…
Tue, Jul 18, 2023
Should I multiply the results obtained by the dilution factor?
Yes, the dilution factor must be applied to the calculated data to get the correct amount of biological marker present…
Tue, Jul 18, 2023
What is the minimum sample volume for using the kit?
The assay requires 100 µL of diluted sample per well. The amount needed, therefore, depends on the samples themselves and…
Tue, Jul 18, 2023
Do you check for cross-reactivity with other steroids?
Yes, cross-reactivities for a number of related steroid compounds were determined and are listed in the kit insert.
Tue, Jul 18, 2023