
For Quantitation Limit (QL) with E. coli Host Cell Protein ELISA kit, ENZ-KIT127, why is 10 ng/mL chosen for precision and accuracy?
A measurable slope is observed between 10 and 30 ng/mL concentrations and anything below 10 ng/mL cannot be easily validated.
Tue, Jul 18, 2023

What is the recommended sample volume to use for the TFA extraction protocol with Substance P ELISA kit, ADI-900-018A ?
Frequently used sample volumes for Substance P ELISA kit range from 250 µl – 5ml. Depending on the type of…
Tue, Jul 18, 2023

What is the percent error range set for newly manufactured lots of E. coli Host Cell Protein ELISA kit, ENZ-KIT127?
We do not have defined cut-offs for % error when looking at the performance of new plate lots. Quality control…
Tue, Jul 18, 2023

Why is boiling samples necessary with Protein A ELISA kit, ADI-900-057?
Samples containing IgG requires boiling to dissociate Protein A in the sample from IgG
Tue, Jul 18, 2023

If a speed vac is not available to dry down samples, how can samples be dried while working with Bradykinin ELISA kit, ADI-900-206?
Vacuum centrifugation (e.g. SpeedVac) is usually the preferred option when a large number of samples has to be dried. In…
Tue, Jul 18, 2023

Can the results form PEGylated protein ELISA kit, ADI-900-213 be used interpret as potential source of allergy to Polyethylene Glycol?
Enzo PEGylated protein ELISA kit, ADI-900-213-0001 is designated for research use only (RUO) and can not be used in diagnostics.
Tue, Jul 18, 2023

Which proteasome subunit does the capture antibody in Proteasome ELISA kit, BML-PW0575 bind to?
Proteasome ELISA Kit, BML-PW0575 is an immunometric sandwich ELISA. The capture antibody is Alpha 6 subunit, 20S proteasome, mouse monoclonal…
Tue, Jul 18, 2023

Do we have a protocol to lyse yeast cells to quantify intracellular cAMP concentrations with Direct cAMP ELISA kit, ADI-900-066A?
We provide 0.1 M HCl to lyse cells and tissue. We do not have a protocol for lysing yeast cells.
Tue, Jul 18, 2023

At which step should the PMSF and Protease Inhibitor cocktail be added to the sample for Bradykinin ELISA kit, ADI-900-206?
As indicated in page 5 of user manual (Step 4), protease inhibitor cocktail and PMSF should be added during the…
Tue, Jul 18, 2023

How long does it take to dry down samples after blood is mixed with ice cold ethanol for Bradykinin ELISA, ADI-900-206?
Precise time to dry down depends on the efficiency of the SpeedVac. Usually it would take 2-4 hrs to completely…
Tue, Jul 18, 2023