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Cellular Analysis
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Can tissue be used with your cellular senescence activity assay (ENZ-KIT129)?
Tissues can be used with this assay. They will need to be re-suspended in the 1X cell lysis buffer provided…
Tue, Jul 18, 2023
How long can the cells be saved until analysis by flow cytometry when using the Cellular senescence live cell analysis assay (ENZ-KIT130)?
Analyzing the results within a few hours is recommended because this assay uses live cells. If the cells lyse, the dye…
Tue, Jul 18, 2023
Does the Hypoxia Red Detection Reagent provided with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, retain the red stain during oxygenation?
The hypoxia dye in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, does not fade during oxygenation.
Tue, Jul 18, 2023
How long does the fluorescence last post-staining with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
Storing the samples stained with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, protected from exposure to direct light would help…
Tue, Jul 18, 2023
Is the peptide substrate coated on the microtiter plate present in the ADI-EKS-390A kit specific for PKA?
No, the peptide substrate in the PKA kinase activity kit (ADI-EKS-390A) is not specific to PKA and therefore it can…
Tue, Jul 18, 2023
What is the half-life of Hypoxia Red Detection Reagent and Oxidative Stress Detection Reagent in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
We have not determined the half-life of the dyes provided with the ROS-ID® Hypoxia/Oxidative stress detection kit (ENZ-51042) but both…
Tue, Jul 18, 2023
Do the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) require a reading by FACS or could a fluorescence reader such as Envision reader be used?
The the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for Flow Cytometry and Microscopy. This does not…
Tue, Jul 18, 2023
Have the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 been tested and used in clinical trial samples such as PBMC, bone marrow samples?
We have not used EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) with clinical samples and we are not aware if…
Tue, Jul 18, 2023
Does the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 kits work with frozen samples?
The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for live cell analysis. If cells are healthy upon…
Tue, Jul 18, 2023