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Cellular Analysis
What is the purpose of the cell pre-treatment solution in ENZ-KIT130?
There are two main types of b-galactosidases in senescent cells: SA-b-galactosidase and lysosomal b-galactosidase. The latter can only be detected…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay(ENZ-KIT130), how long does the fluorescence last?
The fluorescence will last for several hours to one day.
Tue, Jul 18, 2023
How long can the cells be saved until analysis by flow cytometry when using the Cellular senescence live cell analysis assay (ENZ-KIT130)?
Analyzing the results within a few hours is recommended because this assay uses live cells. If the cells lyse, the dye…
Tue, Jul 18, 2023
Does the Hypoxia Red Detection Reagent provided with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, retain the red stain during oxygenation?
The hypoxia dye in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, does not fade during oxygenation.
Tue, Jul 18, 2023
What is the half-life of Hypoxia Red Detection Reagent and Oxidative Stress Detection Reagent in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
We have not determined the half-life of the dyes provided with the ROS-ID® Hypoxia/Oxidative stress detection kit (ENZ-51042) but both…
Tue, Jul 18, 2023
Is the peptide substrate coated on the microtiter plate present in the ADI-EKS-390A kit specific for PKA?
No, the peptide substrate in the PKA kinase activity kit (ADI-EKS-390A) is not specific to PKA and therefore it can…
Tue, Jul 18, 2023
How long does the fluorescence last post-staining with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
Storing the samples stained with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, protected from exposure to direct light would help…
Tue, Jul 18, 2023
Do the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) require a reading by FACS or could a fluorescence reader such as Envision reader be used?
The the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for Flow Cytometry and Microscopy. This does not…
Tue, Jul 18, 2023
Can a multiplex assay be set up for testing three pumps at a time with The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is not possible to differentiate the pumps in a single assay with the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029,…
Tue, Jul 18, 2023
Can different colored dyes be used to measure a different parameter with eThe eFluxx-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is possible to use a dye which would not have spectral overlap with the EFLUXX-ID® dyes (ENZ-51029, ENZ-51030) and…
Tue, Jul 18, 2023