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Cellular Analysis
Are ENZ-KIT129 and ENZ-KIT130 compatible with any cell line?
The SA-b-galactosidase is a universal marker for any senescent cell and this assay is not cell type-specific.
Tue, Jul 18, 2023
Why does the cell pre-treatment solution in ENZ-KIT130 appear to be toxic to my cells?
The cell pre-treatment solution is non-toxic to cells and should not induce cell death. However, some cell types do experience…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay(ENZ-KIT130), how long does the fluorescence last?
The fluorescence will last for several hours to one day.
Tue, Jul 18, 2023
Does the Hypoxia Red Detection Reagent provided with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, retain the red stain during oxygenation?
The hypoxia dye in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, does not fade during oxygenation.
Tue, Jul 18, 2023
How long does the fluorescence last post-staining with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
Storing the samples stained with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, protected from exposure to direct light would help…
Tue, Jul 18, 2023
What is the half-life of Hypoxia Red Detection Reagent and Oxidative Stress Detection Reagent in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
We have not determined the half-life of the dyes provided with the ROS-ID® Hypoxia/Oxidative stress detection kit (ENZ-51042) but both…
Tue, Jul 18, 2023
Is the peptide substrate coated on the microtiter plate present in the ADI-EKS-390A kit specific for PKA?
No, the peptide substrate in the PKA kinase activity kit (ADI-EKS-390A) is not specific to PKA and therefore it can…
Tue, Jul 18, 2023
Do the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) require a reading by FACS or could a fluorescence reader such as Envision reader be used?
The the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for Flow Cytometry and Microscopy. This does not…
Tue, Jul 18, 2023
Does the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 kits work with frozen samples?
The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for live cell analysis. If cells are healthy upon…
Tue, Jul 18, 2023
Have the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 been tested and used in clinical trial samples such as PBMC, bone marrow samples?
We have not used EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) with clinical samples and we are not aware if…
Tue, Jul 18, 2023