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Cellular Analysis
How many cells should I use with :your Cellular senescence activity assay?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Under what conditions were the cells subjected to create hypoxic conditions with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042? Were the cells grown under 95% N2 5 % CO2 ?
When validating the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, cells were grown in 95% nitrogen and also validated with either…
Tue, Jul 18, 2023
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Are collagen-coated plates compatible with your cellular senescence activity assay (ENZ-KIT129)?
Collagen will not interfere with this assay. You can, therefore, grow your cells on collagen-coated plates.
Tue, Jul 18, 2023
Are ENZ-KIT129 and ENZ-KIT130 compatible with any cell line?
The SA-b-galactosidase is a universal marker for any senescent cell and this assay is not cell type-specific.
Tue, Jul 18, 2023
Why does the cell pre-treatment solution in ENZ-KIT130 appear to be toxic to my cells?
The cell pre-treatment solution is non-toxic to cells and should not induce cell death. However, some cell types do experience…
Tue, Jul 18, 2023
Can ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, be used for microplate assay format?
ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, has not been validated in microplate format. The kit is validated for fluorescence microscopy and…
Tue, Jul 18, 2023
Does tissue homogenates or cell lysate work with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, is designed for functional and real time detection in live cells. The kit would…
Tue, Jul 18, 2023
Can tissue be used with your cellular senescence activity assay (ENZ-KIT129)?
Tissues can be used with this assay. They will need to be re-suspended in the 1X cell lysis buffer provided…
Tue, Jul 18, 2023
What is the purpose of the cell pre-treatment solution in ENZ-KIT130?
There are two main types of b-galactosidases in senescent cells: SA-b-galactosidase and lysosomal b-galactosidase. The latter can only be detected…
Tue, Jul 18, 2023