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Cellular Analysis
Could you recommend a control compound Calcineurin inhibitor to use with the Calcineurin Phosphatase assay kit, BML-AK804?
In the kit insert for our Calcineurin Phosphatase assay kit, BML-AK804, we list several inhibitors that were screened with the…
Tue, Jul 18, 2023
The Calcineurin Phosphatase assay kit, BML-AK804, contains ½ volume microplate. What is the advantage of using this type of plate instead of a standard 96 well plate?
We use 1/2 volume plates for the Calcineurin Phosphatase assay kit (BML-AK804) because a longer path length improves accuracy for…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay (ENZ-KIT130), what is the best incubation time, 4 hours or overnight?
An overnight incubation will give better results, especially with cells that may have lower SA-b-galactosidase activity.
Tue, Jul 18, 2023
Can your cellular senescence live cell analysis assay (ENZ-KIT130) be used with non-adherent cells?
ENZ-KIT130 can be used on any mammalian cells, including suspension cells. The only protocol modification required is to spin down…
Tue, Jul 18, 2023
Do I need to incubate the cells in an incubator with 5% CO2 when using Cellular senescence live cell analysis assay (ENZ-KIT130)?
ENZ-KIT130 is performed on live cells, so the incubation steps should be performed under their normal growth conditions. In other…
Tue, Jul 18, 2023
How is your senescence live cell analysis assay any different from an X-Gal assay?
This assay detects the same activity as X-Gal but it is more sensitive. If a visual difference is detected with…
Tue, Jul 18, 2023
Can I fix my cells after staining with your cellular senescence live cell analysis assay (ENZ-KIT130)?
This is actually live cell assay and regretfully, its behavior in presence of a fixative solution cannot be predicted.
Tue, Jul 18, 2023
What is the detection mechanism used in your cellular senescence activity assay (ENZ-KIT129)?
Concerning the components of the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, the hypoxia detection reagent is relatively stable and can…
Tue, Jul 18, 2023
Is the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, specific for live cell imaging? Can cells be fixed after staining?
Yes, the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, is validated on living cells only and tt would not be possible…
Tue, Jul 18, 2023
How is the SA-beta-galactosidase measured with your cellular senescence activity assay (ENZ-KIT129)?
This assay uses a fluorometric substrate to detect SA-b-galactosidase activity in cell lysates. The buffers included in this kit maintain…
Tue, Jul 18, 2023