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Cellular Analysis
Does DAF-2 react with ascorbic acid?
X. Zhang, et al. (2002) reported that DAF-2 reacted with dehydroascorbic acid (DHA) and ascorbic acid (AA) to generate fluorescent…
Tue, Jul 18, 2023
What is the difference between storing a product at -70°C and -80°C?
Products with instructions to be stored at either of these temperatures can be stored at =-70°C. Storage at -70°C or…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay (ENZ-KIT130), what is the best incubation time, 4 hours or overnight?
An overnight incubation will give better results, especially with cells that may have lower SA-b-galactosidase activity.
Tue, Jul 18, 2023
How is your senescence live cell analysis assay any different from an X-Gal assay?
This assay detects the same activity as X-Gal but it is more sensitive. If a visual difference is detected with…
Tue, Jul 18, 2023
Can I fix my cells after staining with your cellular senescence live cell analysis assay (ENZ-KIT130)?
This is actually live cell assay and regretfully, its behavior in presence of a fixative solution cannot be predicted.
Tue, Jul 18, 2023
Do I need to incubate the cells in an incubator with 5% CO2 when using Cellular senescence live cell analysis assay (ENZ-KIT130)?
ENZ-KIT130 is performed on live cells, so the incubation steps should be performed under their normal growth conditions. In other…
Tue, Jul 18, 2023
Can your cellular senescence live cell analysis assay (ENZ-KIT130) be used with non-adherent cells?
ENZ-KIT130 can be used on any mammalian cells, including suspension cells. The only protocol modification required is to spin down…
Tue, Jul 18, 2023
What is the stability and shelf life of Hypoxia/Oxidative Stress Detection Mix, when working with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Are collagen-coated plates compatible with your cellular senescence activity assay (ENZ-KIT129)?
Collagen will not interfere with this assay. You can, therefore, grow your cells on collagen-coated plates.
Tue, Jul 18, 2023
Can ENZ-KIT130 be used with cells expressing GFP?
ENZ-KIT130 is not recommended for use on GFP-expressing cells because of the emission spectra overlap between GFP and the fluorogenic…
Tue, Jul 18, 2023