Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Cellular Analysis
In the manual for the Calcineurin cellular activity assay kit, BML-AK816, you recommend using 5,000-50,000 cells per assay point. How much lysis buffer should be used to prepare the samples?
it is easier to dilute samples than to concentrate them. In order to prepare cells for use with Calcineurin cellular…
Tue, Jul 18, 2023
What is the difference between storing a product at -70°C and -80°C?
Products with instructions to be stored at either of these temperatures can be stored at =-70°C. Storage at -70°C or…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay (ENZ-KIT130), what is the best incubation time, 4 hours or overnight?
An overnight incubation will give better results, especially with cells that may have lower SA-b-galactosidase activity.
Tue, Jul 18, 2023
How is your senescence live cell analysis assay any different from an X-Gal assay?
This assay detects the same activity as X-Gal but it is more sensitive. If a visual difference is detected with…
Tue, Jul 18, 2023
Can I fix my cells after staining with your cellular senescence live cell analysis assay (ENZ-KIT130)?
This is actually live cell assay and regretfully, its behavior in presence of a fixative solution cannot be predicted.
Tue, Jul 18, 2023
Do I need to incubate the cells in an incubator with 5% CO2 when using Cellular senescence live cell analysis assay (ENZ-KIT130)?
ENZ-KIT130 is performed on live cells, so the incubation steps should be performed under their normal growth conditions. In other…
Tue, Jul 18, 2023
Can your cellular senescence live cell analysis assay (ENZ-KIT130) be used with non-adherent cells?
ENZ-KIT130 can be used on any mammalian cells, including suspension cells. The only protocol modification required is to spin down…
Tue, Jul 18, 2023
How is the SA-beta-galactosidase measured with your cellular senescence activity assay (ENZ-KIT129)?
This assay uses a fluorometric substrate to detect SA-b-galactosidase activity in cell lysates. The buffers included in this kit maintain…
Tue, Jul 18, 2023
How many cells should I use with :your Cellular senescence activity assay?
The cell number used with this assay can vary depending on the percentage of cells expected to be senescent. A…
Tue, Jul 18, 2023
Why does the cell pre-treatment solution in ENZ-KIT130 appear to be toxic to my cells?
The cell pre-treatment solution is non-toxic to cells and should not induce cell death. However, some cell types do experience…
Tue, Jul 18, 2023