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Cellular Analysis
What is the mechanism of the Golgi-ID™ Green assay kit
The Green detection reagent included in the kit ENZ-51028 is a fluorescent sphingolipid analog that is metabolized by cells and…
Tue, Jul 18, 2023
What is the difference between PROTEOSTAT® Aggresome detection kit (ENZ-51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023)?
Both our PROTEOSTAT® Aggresome detection kit (ENZ51035) and PROTEOSTAT® Protein Aggregation Assay (ENZ-51023) rely on the properties of our PROTEOSTAT®…
Tue, Jul 18, 2023
How long should I incubate my LEADING LIGHT® Wnt reporter cells after adding the luciferase substrate?
Signals should be read 10 minutes after adding the luciferase substrate with a multichannel pipette. The signal is stable enough…
Tue, Jul 18, 2023
Do I need to use the LEADING LIGHT® Wnt cell line medium pack?
The LEADING LIGHT® Wnt cell line medium pack (ENZ-60003) is made of a cell freezing medium, a cell growth medium…
Tue, Jul 18, 2023
How can I stop the LEADING LIGHT® Wnt reporter cells from clumping?
It is important to remove the Cell Growth Medium entirely before adding trypsin to the cells. Usually, clumping is caused…
Tue, Jul 18, 2023
Is the LEADING LIGHT® Wnt reporter cell line stable?
The LEADING LIGHT® Wnt reporter cell line is a stably transfected cell line. Although stable, the cell line will start…
Tue, Jul 18, 2023
Can I use the LEADING LIGHT® Wnt reporter cells straight from the liquid nitrogen?
We would advise against using the cells from the liquid nitrogen. Cells need to be in their exponential growing phase…
Tue, Jul 18, 2023
What information can you share about the LEADING LIGHT® Wnt reporter cell line?
The LEADING LIGHT® Wnt reporter cell line is a genetically engineered 3T3 mouse fibroblast cell line that contains a luciferase…
Tue, Jul 18, 2023
Can you share the recipe of the Cell Growth Medium?
This information is considered to be proprietary and we cannot disclose the actual recipe. It contains additives and nutrients. These…
Tue, Jul 18, 2023
Why was Wnt3a chosen as agonist for the LEADING LIGHT® Wnt reporter cell line?
Wnt3a is the most commonly studied agonist of the canonical Wnt signaling pathway and that is why it was selected…
Tue, Jul 18, 2023