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Cellular Analysis
What is the likely explanation for lack of staining cells with NUCLEAR-ID® Red DNA stain, ENZ-52406 for FACS application, when 250-fold and 500-fold dilution and incubation at 37°C for 30 minutes were tried?
It is important to check cell density and to ensure that cell concentration is 1 x 105 to 5 x…
Tue, Jul 18, 2023
How do MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51022) work?
MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51002) appear to target the inner mitochondrial membrane in mammalian cells, which…
Tue, Jul 18, 2023
Have any of Enzo Live Cell Fluorescent probes been validated on 3D spheroid culture?
While we did not specifically tested our Live Cell Analysis products on 3D models, we do have a few application…
Tue, Jul 18, 2023
Is it possible to multiplex MITO-ID® Membrane Potential Detection Kit, ENZ-51018 with NUCLEAR-ID® Blue/Red Cell Viability Reagent (GFP-CERTIFIED®), ENZ-53005?
MITO-ID/NUCLEAR-ID (ENZ-51018/ENZ-53005) multiplex assay have been multiplexed in a 3D spheroid cultures of iPSC-derived human hepatocytes (iCell Hepatocytes 2.0) assay…
Tue, Jul 18, 2023
Would COX activity assay kit, ADI-907-003 work with human plasma and mouse cell lysate samples?
COX activity assay kit, ADI-907-003 https://www.enzolifesciences.com/fileadmin/files/manual/ADI-907-003_insert.pdf is designed for the quantitative determination of COX activity in biological samples. However, we…
Tue, Jul 18, 2023
How specific are colorimetric/fluorogenic substrates in Matrix Metalloproteinase (MMP) drug discovery kits?
Several MMPs cleave both BML-P277-9090 substrate (OMNIMMP™ RED fluorogenic substrate peptide), BML-P278-9090 substrate (MPP-3 Fluorogenic Substrate peptide) and colorimetric substrate…
Tue, Jul 18, 2023
Can PAP-AMC, Tyrosinase substrate, BML-EI405 function in live cell assay?
PAP-AMC, Tyrosinase substrate, BML-EI405 has been used in vitro assays. We do not have information if this substrate works efficiently…
Tue, Jul 18, 2023
What is the formulation of BIOMOL® Green (BML-AK111)?
The formulation of BIOMOL® (BML-AK111) Green is proprietary.
Tue, Jul 18, 2023
Does ORGANELLE-ID-RGB® III assay kit, ENZ-51032, bind to a target protein in ER and Golgi compartment?
The Golgi-green dye in the ORGANELLE-ID-RGB® III assay kit (ENZ-51032) is a fluorescent lipid analog which is transported directly into…
Tue, Jul 18, 2023
Can Polymount be used with a coverslip to image fixed cells stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035? Is the fluorescence stable to image following day?
Solvents should be avoided for imaging specimens stained with PROTEOSTAT® Aggresome detection kit, ENZ-51035, as this is known to cause…
Tue, Jul 18, 2023