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Aggregate Detection
If the test protein is a variant with near identical amino acid sequence with the exception of one residue, is one peak expected ?
There may be a possibility of different conformations of the same protein aggregating at different temperatures. The sample could have…
Tue, Jul 18, 2023
Are there any limitations regarding particle size of the aggregates that can be detected with the ProteoStat® dye?
The ProteoStat® dye detects in the subvisible to visible range from ~ 50 µm to 1 mm.
Tue, Jul 18, 2023
Is the binding of the protein to the ProteoStat® detection dye reversible?
The interaction between the ProteoStat® dye and the protein aggregates is non-covalent. So, in theory it is a reversible interaction.…
Tue, Jul 18, 2023
What are the critical points to pay attention to during preparation of ß-Lactoglobulin solution?
During preparation of ß-Lactoglobulin solution, the following points should be noted: The ß-Lactoglobulin Control is supplied as a lyophilized powder…
Tue, Jul 18, 2023
Would the aggregation temperature as determined using ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, be the same as determined using the Thermofluor technique?
In Thermofluor technique, SYPRO Orange, an environment-sensitive dye, is used to measure the unfolding of the protein as the temperature…
Tue, Jul 18, 2023
What are the applications for ProteoStat® Thermal shift stability assay kit, ENZ-51027?
The ProteoStat® Thermal Shift Stability Assay kit (ENZ-51027) is used for direct monitoring of protein aggregation arising from a thermally-induced…
Tue, Jul 18, 2023
What are the differences between ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027, Protein Aggregation Assay kit, ENZ-51023, and ProteoStat® Aggresome Detection kit, ENZ-51035?
ProteoStat® dyes belongs to the class of molecular rotor dyes. They are non-fluorescent in solution due to free rotation around…
Tue, Jul 18, 2023
How different is ProteoStat® TS Detection Reagent in ENZ-51027 from ProteoStat® Detection Reagent in ENZ-51023?
ProteoStat®TS Detection Reagent in ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, and ProteoStat®Detection Reagentin ProteoStat® Protein Aggregation Assay, ENZ-51023, are…
Tue, Jul 18, 2023
Can the ProteoStat® TS Detection Reagent in the thermal shift stability assay kit, ENZ-51027, be substituted with the detection reagent from the ProteoStat® Protein Aggregation Assay, ENZ-51023?
Theoretically, the two dye reagents can be interchanged but this would require some optimization.
Tue, Jul 18, 2023
What is the recommended storage condition for ß-Lactoglobulin solution?
The solution of ß-Lactoglobulin can be stored at 4°C for up to a month. For long term storage, it is…
Tue, Jul 18, 2023