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Knowledge Base
What is the concentration of the PGE2-Alkaline phosphatase conjugate in the PGE2 ELISA kit, ADI-900-001?
Concentration of the PGE2-AP conjugate is proprietary and can't be revealed.
Tue, Jul 18, 2023
How does PATHO-GENE® HPV Type 11 Probe, ENZ-GEN151 work?
The ENZO PATHO-GENE® HPV Type 11 Probe (ENZ-GEN151) is a biotin-labeled DNA probe designed to hybridize to HPV Type 11 DNA sequences. The…
Tue, Jul 18, 2023
What is the suggestion for using part of the ADI-900-057, Protein A ELISA kit then re-using the remainder of the kit at a later date?
In general, we would not advise holding the entire kit at 5-6 hours at ambient temperature if this can be…
Tue, Jul 18, 2023
Can a rough estimate antibody concentration of a-Gal Epitope (Gala1-3Galß1-4GlcNAc-R) monoclonal antibody (M86), ALX-801-090-1 be stated?
The supernatant of ammonium sulfate precipitate hybridoma tissue culture supernatant for The a-Gal epitope monoclonal antibody (ALX-801-090-1) is concentrated and…
Tue, Jul 18, 2023
How is Melanoma marker (human) monoclonal antibody (HMB45), ENZ-C34930 purified from ascites?
Melanoma marker (human) monoclonal antibody (HMB45) (ENZ-C34930) is purified following ammonium sulphate precipitation of ascites. Supernatant is further purified on…
Tue, Jul 18, 2023
What does Standard Diluent refer to in several ELISA user manual when cell culture supernatant is used?
Standard diluent refers to either the tissue culture media (a.k.a. non-conditioned media) or the assay buffer provided. So, the supernatant…
Tue, Jul 18, 2023
What is the concentration of a-Gal Epitope (Gala1-3Galß1-4GlcNAc-R) monoclonal antibody (M86), ALX-801-090-1?
The a-Gal epitope monoclonal antibody (ALX-801-090-1) is offered as partially purified ammonium sulfate precipitate tissue culture supernatant. The volume provided…
Tue, Jul 18, 2023
What volume of recombinant PKC or cell extracts should be used with ADI-EKS-420A?
In experiments with mouse brain lysates using the PKC kinase activity kit (ADI-EKS-420A), we found that 0.02 – 2µg of…
Tue, Jul 18, 2023
Please provide the amino acid sequence of the FLAG tag for Irisin (human),(recombinant)(active), ADI-908-307.
FLAG® is a 8 amino acid sequence DYKDDDDK. In our Irisin (human), (recombinant) (active) (ADI-908-307) amino acids sequence 32-143 of…
Tue, Jul 18, 2023
What are important critical factors for success while working with Protein A ELISA kit, ADI-900-057
Following are two critical factors to be considered: 1. If samples contain IgG, standards must be boiled. 2. Diluted standards…
Tue, Jul 18, 2023