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Buffer salts in 10x PBS-T wash buffer in E. coli host cell protein ELISA kit, ENZ-KIT127 has precipitated upon storage at 4°C. Is this usual and how to dissolve the salts?
On occasions, buffer salts precipitate out of solution at 4°C. Warming the bottle containing buffer at 37°C and frequent vortexing would…
Tue, Jul 18, 2023
What is the difference is between Microycystin-LR (Analytical Standard), ALX-350-431 and Microcystin-LR, ALX-350-012?
Microycystin-LR (Analytical Standard), ALX-350-431-C010 and Microcystin-LR, ALX-350-012 differ in percentage purity. Microcystin-LR (Analytical Standard), ALX-350-431-C010 is =99% (HPLC). Microcystin-LR, ALX-350-012…
Tue, Jul 18, 2023
What is the suggestion for using part of the ADI-900-057, Protein A ELISA kit then re-using the remainder of the kit at a later date?
In general, we would not advise holding the entire kit at 5-6 hours at ambient temperature if this can be…
Tue, Jul 18, 2023
What volume of recombinant PKC or cell extracts should be used with ADI-EKS-420A?
In experiments with mouse brain lysates using the PKC kinase activity kit (ADI-EKS-420A), we found that 0.02 – 2µg of…
Tue, Jul 18, 2023
What kind of protein A ligand is the standard in Protein A ELISA kit, ADI-900-057?
Protein A Standard in Protein A ELISA kit (ADI-900-057) is purified recombinant Protein A expressed in E.coli.
Tue, Jul 18, 2023
Can a rough estimate antibody concentration of a-Gal Epitope (Gala1-3Galß1-4GlcNAc-R) monoclonal antibody (M86), ALX-801-090-1 be stated?
The supernatant of ammonium sulfate precipitate hybridoma tissue culture supernatant for The a-Gal epitope monoclonal antibody (ALX-801-090-1) is concentrated and…
Tue, Jul 18, 2023
Can the Absorbance 96 plate reader be operated with own/custom software?
We can provide you with the necessary serial interface commands for operation to establish your own software. Please contact us…
Tue, Jul 18, 2023
What does Standard Diluent refer to in several ELISA user manual when cell culture supernatant is used?
Standard diluent refers to either the tissue culture media (a.k.a. non-conditioned media) or the assay buffer provided. So, the supernatant…
Tue, Jul 18, 2023
What is the difference between Calcineurin Phosphatase assay kit, BML-AK804 and Calcineurin cellular activity assay kit, BML-AK816?
Calcineurin Phosphatase assay kit (BML-AK804) is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. This kit comes with…
Tue, Jul 18, 2023
Can the Absorbance 96 plate reader be included in an automated workflow?
This may depend on your setup. If your setup is equipped with robotic arms, actuators, etc. that can mimic the…
Tue, Jul 18, 2023