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Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions for the Oxytocin ELISA kit?
The amount of sample required and the dilutions needed for the assay depend upon the level of oxytocin in the…
Tue, Jul 18, 2023
Can I have further details about the HeLa nuclear extract included in your HDAC activity assay kits?
The HeLa nuclear extract is prepared by high salt extraction of HeLa nuclei using a modified protocol from the ones…
Tue, Jul 18, 2023
What is the enzyme composition of the HeLa nuclear extract included in your HDAC activity assay kits?
The HeLa nuclear extract is unpurified and therefore contains possibly all enzymes that can be found in the nucleus of…
Tue, Jul 18, 2023
When running an HDAC activity assay, what is the appropriate stock and working concentrations for my test compounds?
It is not possible for us to predict the appropriate stock solution or final assay concentrations for your test compounds…
Tue, Jul 18, 2023
What are the MW of KillerTRAIL™ and SuperKillerTRAIL™?
Enzo Life Sciences’ KillerTRAIL™ and SuperKillerTRAIL™ monomers have a calculated MW of 24,144 Da and 24,350 Da, respectively. Under reducing…
Tue, Jul 18, 2023
Can I use the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit to measure SIRT1 activity in my cell/tissue extracts?
BML-AK555 is a drug discovery kit intended for screening potential activators or inhibitors of the recombinant SIRT1 provided in the…
Tue, Jul 18, 2023
What are the excitation and emission spectra of the FLUOR DE LYS® substrate?
The fluorophore is excited with 360nm light and the emitted light is detected at 460nm on a fluorometric microplate reader.…
Tue, Jul 18, 2023
Can you measure the activity of SIRT1 with the FLUOR DE LYS® HDAC fluorometric activity assay kit?
This kit measures the activity of all HDACs provided with a biological sample. Simply changing the substrate from the FLUOR…
Tue, Jul 18, 2023
What is the recommended substrate for the FLUOR DE LYS® SIRT1 fluorometric drug discovery kit?
The FLUOR DE LYS® SIRT1 substrate, the recommended substrate, is provided in the kit. The FLUOR DE LYS® SIRT1 substrate is a…
Tue, Jul 18, 2023