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Is there an alternative protocol for extraction of oxytocin in serum or plasma samples prior to using the Oxytocin ELISA kit?
The procedure described below is an alternative protocol for extraction of oxytocin from aqueous solutions. 1. Add two volumes of ice…
Tue, Jul 18, 2023
What protocol can be used for drying samples for analysis with the Oxytocin and Arg8-Vasopressin ELISA kits?
Samples can be dried using either vacuum centrifugation (e.g., SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023
Do vasopressin kits work on other sample types other than culture supernatants?
Cited sample types include cerebrospinal fluid (CSF), culture supernatants, feces, plasma, saliva, serum, tissue lysate, and urine. In general, the…
Tue, Jul 18, 2023
What is the recommended assay concentration range for KillerTRAIL™ and SuperKillerTRAIL™?
The recommended working dilution range for KillerTRAIL™ is 10-100 ng/ml, and 2-100 ng/ml for SuperKillerTRAIL™. However, adjustment may be necessary for the end-user’s specific…
Tue, Jul 18, 2023
Is this Oxytocin ELISA kit species-independent?
Although novel forms of oxytocin with a single amino acid substitution, which may affect overall antibody binding affinity, have been…
Tue, Jul 18, 2023
What type of column can be used for oxytocin extraction?
Solid phase extraction (SPE) can be performed using reverse phase C18 columns containing 200 mg of sorbent and a volume capacity…
Tue, Jul 18, 2023
Is this Vasopressin ELISA kit species-independent?
The vasopressin molecule sequence does not vary between mammalian species making this kit species-independent. Cited sample origins include dog, human,…
Tue, Jul 18, 2023
Why was my activation of SIRT1 by resveratrol not as strong as expected?
The kinetics of the reaction require some optimization. The optimal incubation time for the reaction can vary depending on concentrations…
Tue, Jul 18, 2023
I am working with rabbit sera. Can I use your Oxytocin ELISA kit for detecting oxytocin in this sample?
Problems may arise when working with rabbit sera because the kit relies on the use of an anti-rabbit IgG. This…
Tue, Jul 18, 2023
Do oxytocin kits work on other sample types other than culture supernatants, milk, plasma or serum?
Cited sample types include cerebral spinal fluids (CSF), feces, plasma, saliva, serum, tissue, and urine.
Tue, Jul 18, 2023