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Knowledge Base
*General Information
The solubility of small molecule products, especially those that are hydrophobic can sometimes be a limiting factor in experimental design.…
Tue, Jul 18, 2023
Low yield and incorporation
Poor labeling often reflects low quality input DNA. This can be due to the presence of contaminants from the purification…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
Can the Cyto-ID® green detection dye be used in conjunction with another dye to differentiate between endosomes and amphisomes?
The Cyto-ID® Red long-term tracer dye, ENZ-51037- inserts into the plasma membrane and later is internalized by endocytosis. Cyto-ID® Red…
Tue, Jul 18, 2023
Poor signal to noise ratio despite good incorporation
Low signal can be caused by inappropriate hybridization conditions (too stringent buffer or excessive temperature) or when the hybridization time…
Tue, Jul 18, 2023
Bright yellow array with reduced magnitude of expected changes
This can be due to insufficient Cot-1 DNA, blocking agents or hybridization and washing conditions that are not stringent enough.
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® Autophagy Detection kit relative to GFP-LC3 transfected cells?
The most significant advantage for Cyto-ID® Autophagy Detection kit is that it's a no-transfection assay. Transfections are transient and there…
Tue, Jul 18, 2023
How stable is the Cyto-ID® Green autophagy dye?
Fluorescent dyes are subject to photobleaching, but this dye is more robust than dyes like fluorescein. Standard handling precautions should…
Tue, Jul 18, 2023
Are the cells fixable after staining with the autophagy kit?
Yes, a protocol for fixing cells is provided in the product manual. Permeabilization of the cellular membrane with detergents, however,…
Tue, Jul 18, 2023
The datasheet that came with my product does not state that material is sterile. Will it contaminate my cells?
Please note that all our products are not sterile unless otherwise indicated on their datasheets. If a product is not…
Tue, Jul 18, 2023