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Can I use my own lysis buffer for the preparation of my cell lysates prior to cAMP/cGMP quantification?
The kit has been optimized for using HCl to both lyse and dilute your standards and samples while used in…
Tue, Jul 18, 2023
What if my cells are not sufficiently lyzed for cAMP/cGMP quantification after the addition of 0.1M HCl?
Cell or tissue lysis can be enhanced by adding 0.1-1% Triton X-100 to 0.1M HCl prior to use. When used…
Tue, Jul 18, 2023
Can I use the cAMP/cGMP ELISA kits for agonism and antagonism studies?
As long as the experimental design is structured so that the end samples can be incorporated into the assay as…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the volume or concentration of antibody for coating?
Altering the volume or concentration of the coating antibody will have an impact on assay performance. Users should validate the…
Tue, Jul 18, 2023
Is the acetylation step really necessary prior to cAMP/cGMP quantification?
Samples should be acetylated if they contain a very low concentration of cAMP or cGMP, or when greater sensitivity is…
Tue, Jul 18, 2023
Can any culture medium be used to measure cAMP/cGMP in culture supernatants with cAMP/cGMP ELISA and cAMP/cGMP Complete ELISA kits?
As indicated in the insert, as long as the standard curve is titrated in the same culture media that the…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP ELISA kit be used with the cGMP ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP Direct ELISA kit be used with the cGMP Direct ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the end…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP complete ELISA kit be used with the cGMP complete ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
Can I detect cytoplasmic cAMP/cGMP with these ELISA kits?
No, these are colorimetric ELISA kits for the quantification of extra-cellular cAMP/cGMP in culture supernatants, serum, and saliva from any…
Tue, Jul 18, 2023