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How many cells should I work with for the quantification of cAMP or cGMP?
The amount of tissue or number of cells to use is entirely dependent on the experimental conditions. It is recommended…
Tue, Jul 18, 2023
How much Assay Buffer is required to reconstitute my serum samples in prior to cAMP/cGMP quantification?
The volume of Assay Buffer needed to reconstitute samples is completely dependent on experimental conditions. We recommend re-suspending 1mL of…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will the type of plates I use for coating affect the assay performance?
We recommend using plates made with high binding plastic, similar to Corning plates (2592). The use of different types of…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the volume or concentration of antibody for coating?
Altering the volume or concentration of the coating antibody will have an impact on assay performance. Users should validate the…
Tue, Jul 18, 2023
Is the acetylation step really necessary prior to cAMP/cGMP quantification?
Samples should be acetylated if they contain a very low concentration of cAMP or cGMP, or when greater sensitivity is…
Tue, Jul 18, 2023
Can any culture medium be used to measure cAMP/cGMP in culture supernatants with cAMP/cGMP ELISA and cAMP/cGMP Complete ELISA kits?
As indicated in the insert, as long as the standard curve is titrated in the same culture media that the…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP ELISA kit be used with the cGMP ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP Direct ELISA kit be used with the cGMP Direct ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the end…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP complete ELISA kit be used with the cGMP complete ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
Can I detect cytoplasmic cAMP/cGMP with these ELISA kits?
No, these are colorimetric ELISA kits for the quantification of extra-cellular cAMP/cGMP in culture supernatants, serum, and saliva from any…
Tue, Jul 18, 2023