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Do I need a luminometer with double injectors for this assay?
Yes. A single injector will not provide good results. You cannot pre-mix the arachidonic acid and substrate to make a…
Tue, Jul 18, 2023
Can I use cell culture samples in the COX Activity assay?
Cell culture samples are compatible with this kit. Since this is an activity assay rather than a quantitative immunoassay, there…
Tue, Jul 18, 2023
In the sPLA2 activity kit (ADI-907-002), how is a Unit defined?
One unit of phospholipase activity is the amount of enzyme needed to hydrolyze 1.0 µmole of L-a-phosphatidylcholine to L-a-lysophosphatidylcholine and…
Tue, Jul 18, 2023
Poor signal observed in the assay
Staining reagent has been exposed to strong light. Protect reagents from exposure to strong light.
Tue, Jul 18, 2023
High fluorescent signal at start of run
Protein formed aggregates before the run started. Use a different buffer or additive. Gain setting is too high. Many thermocyclers…
Tue, Jul 18, 2023
DNA histograms are of poor overall quality.
The key elements in obtaining a histogram of high quality are good sample preparation, correct cell-to dye ratio and proper…
Tue, Jul 18, 2023
No decrease of the fluorescent signal after using a specific inhibitor
Inhibitor concentration is too low or too high. Very low doses of inhibitor may not affect ROS production by inducer.…
Tue, Jul 18, 2023
Low or no fluorescent signal in positive control
Species of interest may react with each other, thus attenuating the expected signal. ROS is a complex interacting system. Blocking…
Tue, Jul 18, 2023
Can I use the COX activity kit to distinguish between COX-1 and -2?
The COX Activity kits measure activity alone, distinguishing between COX-1 or -2. In order to differentiate between the two enzymes,…
Tue, Jul 18, 2023
High fluorescence signal observed in negative control
Sample contains interfering substances. The assay is compatible with commonly used buffers (PBS, Tris, HEPES) and excipients (trehalose and sucrose),…
Tue, Jul 18, 2023