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Knowledge Base
What are the standard QC parameters this kit must accommodate?
For a typical labeling reaction with an input of 500 ng of high quality genomic DNA, the yield should be…
Tue, Jul 18, 2023
Units Conversion Table
Molecule Formula Weight Units Conversion Factor Other Units 2,3-dinor-6-keto-Prostaglandin F1a 342.5 pg/ml 2.92 pmol/l 6-keto-Prostaglandin F1a 370.5 pg/ml 2.7 pmol/l…
Tue, Jul 18, 2023
Immunoassay Good Lab Practices
Always read and follow the product instructions. The information contained in the kit manual is your first source of knowledge…
Tue, Jul 18, 2023
Can DNA labeled with the Enzo Life Sciences kit be used on a Roche NimbleGen™ platform?
Yes, it can be used. It has been noted by cytogenetics labs that Enzo’s CGH labeling kit works perfectly well…
Tue, Jul 18, 2023
Mito-ID cell staining is too strong
Mitochondrial membrane potential dye is too concentrated for the cell type used. Optimize for different cell types by diluting the…
Tue, Jul 18, 2023
Control cells without treatment show low orange signal.
Control cells are not healthy. Extended storage of cells after staining may adversely affect their health >> Use healthy cells…
Tue, Jul 18, 2023
Poor Mito-ID staining observed.
Stained cells may have been exposed to strong light. Mito-ID® MP dye is light sensitive; be sure to protect samples…
Tue, Jul 18, 2023
Healthy cells show very high fluorescence intensity
PMT setting is too high. Lower PMT setting on flow cytometer. Incubation temperature. Incubation with the dye should be at…
Tue, Jul 18, 2023
Chromatin condensation is not observed
Incorrect drug concentration or incubation time for cell type: Make sure the cell preparation protocol generates single cells with minimal…
Tue, Jul 18, 2023
More than two populations appear upon apoptosis induction.
It's the property of the dye. Apoptosis is a multi-stage process and Nuclear-ID green dye can distinguish different levels of…
Tue, Jul 18, 2023