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What is the recommended storage condition for ß-Lactoglobulin solution?
The solution of ß-Lactoglobulin can be stored at 4°C for up to a month. For long term storage, it is…
Tue, Jul 18, 2023
Would any protein work with ProteoStat® Thermal Shift Stability Assay kit, ENZ-51027?
When a protein aggregates in response to thermal stress, then the ProteoStat®dye would detect the aggregates. The exact efficiency of detection…
Tue, Jul 18, 2023
Is the ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, compatible with the food proteins?
Beta-lactoglobulin is the control provided in the kit. In the food industry, protein aggregation is a desired attribute since aggregates impart…
Tue, Jul 18, 2023
How different is ProteoStat® TS Detection Reagent in ENZ-51027 from ProteoStat® Detection Reagent in ENZ-51023?
ProteoStat®TS Detection Reagent in ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, and ProteoStat®Detection Reagentin ProteoStat® Protein Aggregation Assay, ENZ-51023, are…
Tue, Jul 18, 2023
I am having difficulty using my current lot of antibody for my intended application. What should I do?
Please provide as many details about the experiment as possible as well as an example of the data generated. Experiment details…
Tue, Jul 18, 2023
Cells do not exhibit fluorescence after incubation with the detection reagent.
Cell viability is low. Cells should be in log growth phase. Cell samples cannot be kept longer than 6 hours…
Tue, Jul 18, 2023
There are differences in MFI (mean fluorescence intensity) values between cell lines.
Detection reagent accumulation may be influenced by cell size, endogenous esterase activity, etc. Using the inhibitors and calculating the MAF…
Tue, Jul 18, 2023
Inconsistent fluorescence shift using the same cell (irreproducible results).
Inadequate incubation condition. Always use a water bath (not incubator) and ensure temperature of water bath is 37°C.
Tue, Jul 18, 2023
Fluorescence drop upon compound addition
Cells may become dislodged during addition. Decrease the rate of addition or seed fewer cells per well.
Tue, Jul 18, 2023
Chromatin condensation is not observed
Incorrect drug concentration or incubation time for cell type: Make sure the cell preparation protocol generates single cells with minimal…
Tue, Jul 18, 2023