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Is the ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, compatible with the food proteins?
Beta-lactoglobulin is the control provided in the kit. In the food industry, protein aggregation is a desired attribute since aggregates impart…
Tue, Jul 18, 2023
Immunoassay Good Lab Practices
Always read and follow the product instructions. The information contained in the kit manual is your first source of knowledge…
Tue, Jul 18, 2023
Units Conversion Table
Molecule Formula Weight Units Conversion Factor Other Units 2,3-dinor-6-keto-Prostaglandin F1a 342.5 pg/ml 2.92 pmol/l 6-keto-Prostaglandin F1a 370.5 pg/ml 2.7 pmol/l…
Tue, Jul 18, 2023
Can the ProteoStat® TS Detection Reagent in the thermal shift stability assay kit, ENZ-51027, be substituted with the detection reagent from the ProteoStat® Protein Aggregation Assay, ENZ-51023?
Theoretically, the two dye reagents can be interchanged but this would require some optimization.
Tue, Jul 18, 2023
High baseline fluorescence
Growth medium and organic anion transport contribute to high baseline fluorescence. Remove the medium before adding indicator dye to the wells.…
Tue, Jul 18, 2023
Poor Mito-ID staining observed.
Stained cells may have been exposed to strong light. Mito-ID® MP dye is light sensitive; be sure to protect samples…
Tue, Jul 18, 2023
Fluorescence drop upon compound addition
Cells may become dislodged during addition. Decrease the rate of addition or seed fewer cells per well.
Tue, Jul 18, 2023
Control cells without treatment show low orange signal.
Control cells are not healthy. Extended storage of cells after staining may adversely affect their health >> Use healthy cells…
Tue, Jul 18, 2023
Untreated cells have calcium response
Inconsistent DMSO concentration. Ensure that buffer used for the negative control wells has the same final concentration of DMSO as…
Tue, Jul 18, 2023
Well-to-well variation observed
Incorrect dispensing and/or experimental setup parameter used. A fluorescence microplate reader with automatic dispensing should be used for assays requiring precise…
Tue, Jul 18, 2023