When doing a four-color IHC staining, is there a risk that the colors will fade during the process?
It is recommended to optimize with each step in order to avoid fading of each color due to time-lag between…
Tue, Jul 18, 2023
Is the ISH/IHC Peroxidase block solution provided in the MULTIVIEW® PLUS IHC kit (ENZ-KIT181) the same as the IHC tissue primer (ADI-950-234)?
The peroxidase blocker solution is functionally the same as the IHC tissue primer but different in its formulation. The IHC tissue…
Tue, Jul 18, 2023
I plan to perform multiple IHC staining using your MULTIVIEW® PLUS IHC kit. What are your recommendations in case the targets co-localize?
In IHC, designing the experiment correctly is very critical. In the ideal situation, you will label unique proteins or antigens…
Tue, Jul 18, 2023
How can cross-reaction of detection reagents with primary antibodies be avoided using the MULTIVIEW® PLUS kit (ENZ-KIT181)?
Regarding the MULTIVIEW® PLUS (mouse-HRP/mouse-AP) IHC kit (Brown/Green), sufficient washes between antibody, detection, and chromogen steps (3 x 1 minute…
Tue, Jul 18, 2023
Will the HO-1 (h) ELISA cross react with HO-2 and HO-3?
This assay is specific for human HO-1 and will not cross react with human HO-2 and HO-3, the other known…
Tue, Jul 18, 2023
What is the nature of the recombinant HO-1 standard provided in the kit ADI-EKS-800, HO-1 (human), ELISA kit?
The standard is a recombinant human HO-1 (UniProt ID: P09601) expressed in E.coli.
Tue, Jul 18, 2023
How should I handle and store cell lysates if I cannot assay them immediately?
In order to prevent HO-1 degradation it is important to add protease inhibitors in the lysis buffer just prior to…
Tue, Jul 18, 2023
I have always seen the TMB Substrate Solution as a clear solution, but today the solution had significant green-blue tinge. What might have caused this color change?
The color tinge in the TMB substrate is due to contamination by the conjugate. To avoid contamination it is important…
Tue, Jul 18, 2023
Does the product ADI-EKS-800, HO-1 (human), ELISA kit also work for human plasma and serum?
The kit has been validated for the measurement of intracellular HO-1 in cell lysates and tissue extracts and the protocol refers…
Tue, Jul 18, 2023
How long can I use an ELISA kit after expiry?
Once ELISA kits are expired their performance can no longer be guaranteed. However, ELISA kits do not typically stop working entirely on their…
Tue, Jul 18, 2023
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