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Can cell lysates be used to determine protein aggregation with ProteoStat® Aggresome detection reagent?
Working with cell lysates would be challenging, because detergents can cause high background. It is possible to titrate the amount…
Tue, Jul 18, 2023
Can the cells be trypsinized to determine protein aggregation in cell based assay for testing drug responsiveness?
Trypsin can be used with ProteoStat® Aggresome detection kit. Following lysis, detergent can be added to permeabilize the cells. The…
Tue, Jul 18, 2023
Can I measure the levels of 8-OHdG in cell or tissue extracts?
The order of events happening in the cell is that the damaged DNA is modified, halting further transcription. Repair of…
Tue, Jul 18, 2023
Can I use plasma samples with ADI-EKS-350?
Plasma samples were not specifically tested in-house with this assay but there is no reason to believe it would not…
Tue, Jul 18, 2023
Do you have any literature references for normal human 8-OHdG levels using ADI-EKS-350?
Unfortunately, there is no real “gold standard” for comparing normal or expected levels of 8-OHdG. The latter is a rather…
Tue, Jul 18, 2023
I am planning to quantify 8-OHdG in cancer patient samples using ADI-EKS-350. This kit can be used to test urine, serum, and saliva samples. Which sample should I use?
Urine is usually the sample of choice for 8-OHdG when looking at DNA damage; however, which sample may be best…
Tue, Jul 18, 2023
What do I need to do if my antibody concentration is higher than the 50 µg/mL indicated in the manual for ADI-900-057?
If you have samples with an antibody concentration higher than 50 µg/mL, you just need to dilute these samples down to…
Tue, Jul 18, 2023
Does your Protein A ELISA kit (ADI-900-057) detect both “natural” and “unnatural” Protein A?
As is illustrated in the figure included in the data sheet of ADI-900-057, this ELISA kit can detect both native…
Tue, Jul 18, 2023
Can ADI-EKS-750 detect human antibodies against DnaK, an E.coli analog of HSP70?
A similar assay has not been performed and therefore, a positive outcome of such experiment cannot be guaranteed. We would suggest using…
Tue, Jul 18, 2023
Is ADI-EKS-725A specific for HSP70B’ or does it also detect HSP70?
This kit was developed with the sole purpose to detect and quantify HSP70B’ in cell lysates, serum and tissue of…
Tue, Jul 18, 2023