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What is a good positive control to study protein aggregation in cells?
MG132 treated cells would serve as positive control to determine background noise compared to signal along with untreated negative control.
Tue, Jul 18, 2023
I am planning to quantify 8-OHdG in cancer patient samples using ADI-EKS-350. This kit can be used to test urine, serum, and saliva samples. Which sample should I use?
Urine is usually the sample of choice for 8-OHdG when looking at DNA damage; however, which sample may be best…
Tue, Jul 18, 2023
Can I use plasma samples with ADI-EKS-350?
Plasma samples were not specifically tested in-house with this assay but there is no reason to believe it would not…
Tue, Jul 18, 2023
What do I need to do if my antibody concentration is higher than the 50 µg/mL indicated in the manual for ADI-900-057?
If you have samples with an antibody concentration higher than 50 µg/mL, you just need to dilute these samples down to…
Tue, Jul 18, 2023
Can I measure the levels of 8-OHdG in cell or tissue extracts?
The order of events happening in the cell is that the damaged DNA is modified, halting further transcription. Repair of…
Tue, Jul 18, 2023
Would diluting the sample to a lower concentration (e.g. 0.5 mg/mL) change the percentage of aggregate protein?
The percent aggregate (the ratio of aggregate to total protein) will stay the same. However, the concentration of aggregated protein…
Tue, Jul 18, 2023
Is there a step in the protocol of ENZ-51035 at which it is possible to stop and store the samples at 40 C overnight and the protocols can be completed the next day?
It is advisable to stop the protocol if desired before loading the dye. The intensity of the dye decreases over…
Tue, Jul 18, 2023
Does your Protein A ELISA kit (ADI-900-057) detect both “natural” and “unnatural” Protein A?
As is illustrated in the figure included in the data sheet of ADI-900-057, this ELISA kit can detect both native…
Tue, Jul 18, 2023
Can ADI-EKS-750 detect human antibodies against DnaK, an E.coli analog of HSP70?
A similar assay has not been performed and therefore, a positive outcome of such experiment cannot be guaranteed. We would suggest using…
Tue, Jul 18, 2023
Can I detect HSP70 in urine with ADI-EKS-700B, ADI-EKS-715, or ENZ-KIT-101?
Urine is not a sample type which has been validated for these kits, nor are there any publications found citing usage…
Tue, Jul 18, 2023