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Is the kit specific to Endothelin-1?
The antibodies used in this ELISA are highly specific with 100% cross-reactivity to ET-1, 21% to ET-2, 3.6% to ET-3…
Tue, Jul 18, 2023
How long may plasma samples be stored (with EDTA and aprotinin or with EDTA-only)? Has this been tested? Is there some degradation of Endothelin-1 while samples are stored?
Plasma samples collected as directed with EDTA and Aprotinin will be stable for at least 1 month at -80°C without…
Tue, Jul 18, 2023
How essential is the addition of aprotinin when collecting blood samples?
The aprotinin is included to help prevent any degradation of ET-1, but also to prevent any further cleavage of the…
Tue, Jul 18, 2023
Which type of plasma samples can be used with your ADI-900-020A, Endothelin-1 ELISA kit (Heparin, EDTA or Citrate)?
The kit has been validated using EDTA plasma samples. Heparin and citrate plasma were not tested. We generally recommend EDTA plasma for…
Tue, Jul 18, 2023
I bought your ADI-900-020A kit and I just realized that the manual indicates to avoid lipemic samples. My plasma samples are lipemic. Is there a way I can try measuring ET-1 concentration with your kit?
How lipid can potentially interfere in the assay has not specifically been tested, but you might want to consider delipidating your…
Tue, Jul 18, 2023
I plan to use your ADI-900-020A kit to measure Endothelin-1 (ET-1) concentration in cell culture supernatant. Samples contain 10% fetal bovine serum (FBS). Will I need to extract them?
The ET-1 ELISA kit is suitable for use with tissue culture supernatants containing up to 10% FBS. In our spike…
Tue, Jul 18, 2023
Can DAPI be used as counterstaining for the nucleus with the nucleolar green detection kit Nucleolar-ID® Green detection kit for microscopy ENZ-51009 ?
Hoechst 33342 has been shown to work well. DAPI and Hoechst have similar spectral characteristics and either of them should work…
Tue, Jul 18, 2023
Do the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) require a reading by FACS or could a fluorescence reader such as Envision reader be used?
The the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for Flow Cytometry and Microscopy. This does not…
Tue, Jul 18, 2023
Can different colored dyes be used to measure a different parameter with eThe eFluxx-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is possible to use a dye which would not have spectral overlap with the EFLUXX-ID® dyes (ENZ-51029, ENZ-51030) and…
Tue, Jul 18, 2023
Can a multiplex assay be set up for testing three pumps at a time with The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is not possible to differentiate the pumps in a single assay with the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029,…
Tue, Jul 18, 2023