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Do I need to incubate the cells in an incubator with 5% CO2 when using Cellular senescence live cell analysis assay (ENZ-KIT130)?
ENZ-KIT130 is performed on live cells, so the incubation steps should be performed under their normal growth conditions. In other…
Tue, Jul 18, 2023
How is your senescence live cell analysis assay any different from an X-Gal assay?
This assay detects the same activity as X-Gal but it is more sensitive. If a visual difference is detected with…
Tue, Jul 18, 2023
Can I fix my cells after staining with your cellular senescence live cell analysis assay (ENZ-KIT130)?
This is actually live cell assay and regretfully, its behavior in presence of a fixative solution cannot be predicted.
Tue, Jul 18, 2023
How should I determine DNA concentration before labeling with Enzo’s CGH labeling kits?
We recommend determining concentration and purity of the genomic DNA using the nucleic acid module of a NanoDrop UV-VIS Spectrophotometer…
Tue, Jul 18, 2023
Enzo suggests using 50 – 100 µg of Cot-1 DNA per sample when hybridizing the DNA labeled with the CGH labeling kits to Agilent arrays, is that correct?
The suggested amount of Cot-1 DNA in the protocols is correct. However, there is no theoretical reason why Enzo's kits…
Tue, Jul 18, 2023
Is there an alternate stop buffer that can be used with Enzo’s CGH labeling kits?
An EDTA solution may be used to stop the reaction prior to purification instead of the supplied stop buffer.
Tue, Jul 18, 2023
Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
What is the detection mechanism used in your cellular senescence activity assay (ENZ-KIT129)?
Concerning the components of the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, the hypoxia detection reagent is relatively stable and can…
Tue, Jul 18, 2023
Is the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, specific for live cell imaging? Can cells be fixed after staining?
Yes, the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, is validated on living cells only and tt would not be possible…
Tue, Jul 18, 2023
How is the SA-beta-galactosidase measured with your cellular senescence activity assay (ENZ-KIT129)?
This assay uses a fluorometric substrate to detect SA-b-galactosidase activity in cell lysates. The buffers included in this kit maintain…
Tue, Jul 18, 2023