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Could you recommend a control compound Calcineurin inhibitor to use with the Calcineurin Phosphatase assay kit, BML-AK804?
In the kit insert for our Calcineurin Phosphatase assay kit, BML-AK804, we list several inhibitors that were screened with the…
Tue, Jul 18, 2023
The Calcineurin Phosphatase assay kit, BML-AK804, contains ½ volume microplate. What is the advantage of using this type of plate instead of a standard 96 well plate?
We use 1/2 volume plates for the Calcineurin Phosphatase assay kit (BML-AK804) because a longer path length improves accuracy for…
Tue, Jul 18, 2023
Are Enzo’s CGH labeling kits suitable for single nucleotide polymorphism (SNP) analysis?
Our CYTAG® TotalCGH Labeling Kit (ENZ-42674) is perfectly suited for CGH+SNP array analysis. Our CYTAG® CGH (ENZ-42671) and CYTAG® Super…
Tue, Jul 18, 2023
Does genomic DNA need to be fragmented for labeling with Enzo’s CGH labeling kits?
Restriction digestion or fragmentation of the isolated genomic DNA is not required prior to labeling with our CYTAG® CGH (ENZ-42671)…
Tue, Jul 18, 2023
What is the required fragment length of input DNA for labeling with Enzo’s CGH labeling kits?
Comparative genome hybridization (CGH) does not require DANN fragmentation prior to analysis and can be performed with genomic input DANN.…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay (ENZ-KIT130), what is the best incubation time, 4 hours or overnight?
An overnight incubation will give better results, especially with cells that may have lower SA-b-galactosidase activity.
Tue, Jul 18, 2023
Can your cellular senescence live cell analysis assay (ENZ-KIT130) be used with non-adherent cells?
ENZ-KIT130 can be used on any mammalian cells, including suspension cells. The only protocol modification required is to spin down…
Tue, Jul 18, 2023
What is the starting concentration of DNA needed for labeling with the CGH labeling kit for oligo arrays?
We suggest using 0.25 – 2.5 µg of input genomic DNA in a maximum volume of 19 µl, hence a…
Tue, Jul 18, 2023
How can the quality of DNA labeling by Enzo’s CGH labeling kits be analyzed?
We recommend using a NanoDrop UV-VIS Spectrophotometer in the microarray measurement mode to determine yield and dye incorporation. Use 1.5…
Tue, Jul 18, 2023
Do I need to incubate the cells in an incubator with 5% CO2 when using Cellular senescence live cell analysis assay (ENZ-KIT130)?
ENZ-KIT130 is performed on live cells, so the incubation steps should be performed under their normal growth conditions. In other…
Tue, Jul 18, 2023