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Do I need to use the LEADING LIGHT® Wnt cell line medium pack?
The LEADING LIGHT® Wnt cell line medium pack (ENZ-60003) is made of a cell freezing medium, a cell growth medium…
Tue, Jul 18, 2023
How can I stop the LEADING LIGHT® Wnt reporter cells from clumping?
It is important to remove the Cell Growth Medium entirely before adding trypsin to the cells. Usually, clumping is caused…
Tue, Jul 18, 2023
Can components of Enzo’s CGH labeling kits be purchased separately?
Unfortunately, we will not provide components of our CGH labeling kits as our kits are readily packaged and QCed together.…
Tue, Jul 18, 2023
In which cases do you recommend to digest the DNA prior to labeling with Enzo’s CGH labeling kits?
For CGH+SNP arrays, genomic DNA must be digested using a combination of the restriction enzymes AluI and RsaI. For CGH…
Tue, Jul 18, 2023
What will be the effect of decreasing the labeling time with Enzo’s CGH labeling kits below the recommended 4 hours?
For input DNA of good quality and quantity, shorter labeling times down to 2 hours may be sufficient, but the…
Tue, Jul 18, 2023
Apart from the labeling kits, which ancillary reagents does Enzo offer for CGH?
Enzo does provide reagents for restriction, labeling and purification of DNA and Cot DNA. Furthermore, ENZO provides control Human Genomic…
Tue, Jul 18, 2023
Is the LEADING LIGHT® Wnt reporter cell line stable?
The LEADING LIGHT® Wnt reporter cell line is a stably transfected cell line. Although stable, the cell line will start…
Tue, Jul 18, 2023
Can I use the LEADING LIGHT® Wnt reporter cells straight from the liquid nitrogen?
We would advise against using the cells from the liquid nitrogen. Cells need to be in their exponential growing phase…
Tue, Jul 18, 2023
What is the optimal time for hybridization of DNA that was labeled with Enzo’s CGH labeling kits?
Please follow the recommendations of the CGH array manufacturer you are using.
Tue, Jul 18, 2023
When I opened the parcel containing the CGH labeling kit from Enzo, all of the dry ice had disappeared, will it still be good to use?
If the vials in the kit were still frozen when you received them then the product is okay to use.…
Tue, Jul 18, 2023