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Can Excel be used to plot read outs from ELISA? How to calculate reads from ELISA using Excel?
Our recommendation is to analyze ELISA reads by 4P curve fit. Many plate readers do have the ability to perform…
Tue, Jul 18, 2023
Why is 4P curve fit curve recommended for ELISA data analysis and where can I find access to this data analysis software?
For the most accurate results, we normally recommend to use a 4-parameter logistic (4-PL) curve fitting software, as 4-PL fitting…
Tue, Jul 18, 2023
Can you provide an overview of steps in Intracellular Calcium mobilization assay with FLUOFORTE® Calcium assay kit, ENZ-51016 ?
The main steps for the FLUOFORTE® Calcium assay kit (ENZ51016) involves loading cells with dye that fluoresce when exposed to…
Tue, Jul 18, 2023
Is there a requirement for bridging when changing lots of E. coli host cell protein ELISA kit, ENZ-KIT127?
A bridging study is required if this is a part of defined or specified SOP for a facility or a…
Tue, Jul 18, 2023
How long can anti-DNP biotin antibody and streptavidin-HRP be stored once reconstituted for use with Protein Carbonyl ELISA, ALX-850-312?
Anti-DNP-biotin antibody (G) can be stored at -20 °C, for 1-2 weeks and streptavidin-horseradish peroxidase (H) can also be stored…
Tue, Jul 18, 2023
What is the best method to store PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
It is best to freeze PROTEOSTAT® Protein Detection reagent at -20° for long term storage. When many experiments are planned for following…
Tue, Jul 18, 2023
Why do we suggest 96 -well black wall microplate, preferably with a clear bottom for PROTEOSTAT® Protein aggregation assay, ENZ-51023
Black side wall microplates would minimize spillover of RFU reads between the neighboring wells when analyzing data obtained with PROTEOSTAT®…
Tue, Jul 18, 2023
What precautions do we recommend while working with PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
The PROTEOSTAT® Detection Reagent in all these references (ENZ-51023, ENZ-51035 and ENZ-51027) is light sensitive. Avoid direct exposure of the…
Tue, Jul 18, 2023
How are Quality control tests for each lot of E.coli host cell protein ELISA kit, ENZ-KIT127 performed?
Quality control testing is performed by randomly selecting multiple plates from each lot. All lot-specific components are tested together using…
Tue, Jul 18, 2023
How can photobleaching be minimized with PROTEOSTAT® Aggresome detection kit, ENZ-51035?
PROTEOSTAT® dye is light sensitive. Avoid direct exposure of the reagent to intense light. In a microscopy experiment with PROTEOSTAT®…
Tue, Jul 18, 2023