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Oxidative Stress

Trusted Tools for Measuring Oxidative Stress

Origins and Consequences of Oxidative Stress

The term oxidative stress reflects an imbalance in free radical formation within a cell or organism, most commonly in the form of reactive oxygen or nitrogen species (ROS/RNS). ROS/RNS such as superoxide anions, hydroxyl radicals, hydrogen peroxide, nitric oxide, and peroxynitrite originate from a variety of sources including changes in aerobic metabolism, immune activation, UV radiation, heme accumulation, and hypoxia. Failure of the cell’s defense mechanisms to compensate for accumulating insults such as mitochondrial dysfunction, DNA damage, misfolded proteins, and lipid peroxidation can trigger programmed cell death pathways, and has been linked to clinically relevant diseases including cancer, cardiovascular disease, asthma, ischemia, diabetes, and neurodegenerative disease.

Radical Scavengers
SOD and Glutathione Peroxidase Mimetics

Heme Oxygenase ELISAs & Antibodies
Glutathione Peroxidase Assay Kit
p47Phox Antibodies and NADPH Oxidase Inhibitors
Superoxide Dismutase ELISA

HIF-1a & HIF-2a Antibodies
ROS-ID® Hypoxia/Oxidative Stress Detection Kit

8-iso-PGF2a ELISA Kit
ALDETECT™ Lipid Peroxidation Assays
HETE /HODE /LTC ELISA Kits
Lipoxygenase Enzymes
OXI-TEK TBARS Assay Kit

MITO-ID® Membrane potential cytotoxicity kit
MITO-ID® Membrane potential detection kit

Comet SCGE Assay Kit
CYTO-ID® Autophagy Detection Kit
Apoptosis/Necrosis Detection Kit
NUCLEAR-ID® Chromatin Condensation Kit
p53 / Bax & Cytochrome c ELISA Kits
p62 & NBR1 ELISA Kits

HSP70/HSP40/HSP27/HSF-1 Antibodies & ELISAs
Grp75/ Grp78/ Calnexin/ Calreticulin Antibodies
HSP/Chaperone Products

Live Cell, Multiplex Analysis Kits

Accurately Profile Total ROS and Superoxide with Dual-readout Assay

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Multiplex Analysis of Total ROS AND Superoxide

ROS-ID® Total ROS/Superoxide Detection Kit

Non-invasive fluorescent probes allow simultaneous discrimination of total ROS and superoxide in live cells
Profile to distinguish between hydrogen peroxide, peroxynitrite and hydroxyl radicals using included inhibitors
Dyes compatible with major components of tissue culture media (phenol red, FBS and BSA) for microscopy and flow cytometry
Red dye for detection of Nitric Oxide also available (ENZ-51013 / ENZ-51001)

Multiplex, Real-time Analysis of Hypoxia and ROS in Live Cells

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Simultaneously Monitor Hypoxic Status and ROS Formation

ROS-ID® Hypoxia/Oxidative Stress Detection Kit

Sensitive hypoxia dye fluoresces red when converted by nitroreductases
Total ROS dye enables simultaneous detection of hypoxia (red) and ROS (green)
Non-toxic, cell permeable dyes optimized to avoid artifactual fluorescence
Suitable for microscopic or flow cytometric analysis of adherent or suspension cells

Detect & Quantify Biomarkers of Oxidative Stress

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As a trusted manufacturer of thousands of widely cited and thoroughly validated ELISA kits and antibodies, we understand quality means delivering sensitivity, specificity, and consistency. Over 200 immunoassays and 2400 antibodies enable sensitive & specific detection of protein and small molecule biomarkers of oxidative stress. Our assay kits and reagents are some of the most widely cited products for quantification of critical stress-regulating metabolic enzymes and stress-responsive pathways.

DNA Damage (8-OHdG) ELISA Kit

Quantify levels < 1ng/mL
Rapid results in < 2.5 hours
Tested in a variety of biofluids (urine, serum, saliva)
Convenient colorimetric 96-well plate formats

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Rapidly Detect <1 ng/ml 8-OHdG