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LDH Cytotoxicity WST Assay

ENZ-KIT157

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Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity

Homogenous assay using living cells or non-homogenous assay protocol using cell culture supernatant
Stable working solution

The LDH cytotoxicity WST assay is a colorimetric assay kit used to determine cytotoxicity by measuring lactate dehydrogenase activity released from damaged cells.

LDH is a stable cytoplasmic enzyme presented in all types of cells and released into the cell culture medium through damaged plasma membrane.

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LDH Cytotoxicity WST Assay Cytotoxicity — Difference between the Cell Counting Kit-8 (Prod. No. ALX-850-039) and the LDH Cytotoxicity WST Assay. The Cell Counting Kit-8 determines the number of viable cells in cell proliferation and cytotoxicity assays. On the other hand, the LDH Cytotoxicity WST Assay evaluates the cytotoxic level by measuring the amount of LDH released from damaged cells. Assessing the level of cytotoxicity by combining both the Cell Counting Kit-8 Kit and the LDH Cytotoxicity WST Assay can provide researchers with a complete evaluation of cell health.

LDH Cytotoxicity WST Assay mechanism — Mechanism for LDH Cytotoxicity WST Assay

Product Details

Application	Colorimetric detection
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Quantity	500 tests/5ml 2500 tests/5 x 5ml
Technical Info / Product Notes	Principle: LDH catalyzes dehydrogenation of lactate to pyruvate, reducing NAD to NADH. NADH reduces a water soluble tetrazolium salt (WST) in the presence of an electron mediator to produce an orange formazan dye. The amount of formazan dye produced is proportional to the amount of LDH released into the medium, indicating cytotoxicity.

Handling & Storage

Handling	Protect from light. Avoid freeze/thaw cycles.
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Effects of Benzo[k]fluoranthene at Two Temperatures on Viability, Structure, and Detoxification-Related Genes in Rainbow Trout RTL-W1 Cell Spheroids: Esteves, T., Malhão, F., et al.; Toxics 13, 302 (2025), Abstract
Unveiling the Effects of Two Polycyclic Aromatic Hydrocarbons and Two Temperatures on the Trout RTL-W1 Cell Line Expression of Detoxification-Related Target Genes: Vilaça, M., Esteves, T., et al.; J. Xenobiot. 15, 84 (2025), Abstract
The Nrf1 transcription factor is induced by patulin and protects against patulin cytotoxicity: J.J.W. Han, et al.; Toxicology 471, 153173 (2022), Abstract
Bisdemethoxycurcumin-mediated Attenuation of Apoptosis Prevents Gentamicin-induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells: T.Y. Kang, et al.; In Vivo 36, 1095 (2022), Abstract
The role of plasminogen activator inhibitor-2 in pneumococcal meningitis: N.C. Teske, et al.; Acta Neuropathol. Commun. 10, 155 (2022), Abstract
Interruption of Helicobacter pylori-Induced NLRP3 Inflammasome Activation by Chalcone Derivatives: H.R. Choi, et al.; Biomol. Ther. (Seoul) 29, 410 (2021), Application(s): Cytotoxicity after H. pylori infection of THP-1 cells, Abstract
Ethanol extract of Allium fistulosum inhibits development of non-alcoholic fatty liver disease: J.T. Hwang, et al.; Nutr. Res. Pract. 12, 110 (2018), Abstract — Full Text